Determination of scopolamine hydrobromide injection by ion-pair RP-HPLC
- Received Date: 2004-01-10
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Key words:
- scopolamine hydrobromide injection /
- ion-pair RP-HPLC
Abstract: Objective To establish a method to determinate scopolamine hydrobromide injection by ion pair RP-HPLC.Methods The mobile phase of methanol-20mmol/L sodium heptanesulfonate solution acetic acid (50:50:2) and ZORBAX SB-C18 column (4.6×250mm,5μm)was used,the flow rate was 0.9mL/min and the detect wavelength was 225nm.Results The linear range of scopolaminc hydrobromide was 0.1~0.5mg/mL(r=0.9999). The average recovery was 100.15%,RSD was 1.58%.Conclusion The method was simple,rapid and accurate. It can be used to control the quality of scopolamine hydrobromide injection.
Citation: | ZHAO Ping-ge. Determination of scopolamine hydrobromide injection by ion-pair RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2004, (3): 165-166. |