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Volume 23 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2005  >  (6): 339-342

LIU Ye, QI Li-hong, WANG Shuo-feng, ZHANG Yue-fan, GUI Min, ZHANG Min, ZHANG Jun-ping. Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells[J]. Journal of Pharmaceutical Practice and Service, 2005, (6): 339-342.
Citation: LIU Ye, QI Li-hong, WANG Shuo-feng, ZHANG Yue-fan, GUI Min, ZHANG Min, ZHANG Jun-ping. Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells[J]. Journal of Pharmaceutical Practice and Service, 2005, (6): 339-342.
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Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells

  • 1.

    Department of Pharmacy, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350001

  • 2.

    Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2005-09-05
  • Objective To establish a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells. Methods Mouse peritoneal macrophages were primed with calcimycin 10-6mol/L for 8h then elicited by lipopolysaccharides(LPS) 100μg/L for 6h to prepare macrophage conditioned medium(MCM). Proliferative activity and collagen stimulating activity was determined by crystal violet staining assay and[3H]-proline incorporation assay using rat hepatic stellate HSC-T6 cell. Results Serum(0%-20%) and MCM(1:32-1:2) concentration-dependently enhanced HSC-T6 cell proliferation and collagen synthesis. Among IL-1, TNF, EGF, FGF and PDGF, PDGF showed the highest proliferation enhancing activity. TGFβ1 increased HSC-T6 cell collagen synthetic capacity. Conclusion It is feasible to establish an in vitro hepatic fibrosis model selecting HSC-T6 cell proliferation and collagen synthesis as indexes with stimulating factors serum, MCM and cytokines.
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  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells

  • 1. Department of Pharmacy, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350001
  • 2. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2005-09-05

Abstract: Objective To establish a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells. Methods Mouse peritoneal macrophages were primed with calcimycin 10-6mol/L for 8h then elicited by lipopolysaccharides(LPS) 100μg/L for 6h to prepare macrophage conditioned medium(MCM). Proliferative activity and collagen stimulating activity was determined by crystal violet staining assay and[3H]-proline incorporation assay using rat hepatic stellate HSC-T6 cell. Results Serum(0%-20%) and MCM(1:32-1:2) concentration-dependently enhanced HSC-T6 cell proliferation and collagen synthesis. Among IL-1, TNF, EGF, FGF and PDGF, PDGF showed the highest proliferation enhancing activity. TGFβ1 increased HSC-T6 cell collagen synthetic capacity. Conclusion It is feasible to establish an in vitro hepatic fibrosis model selecting HSC-T6 cell proliferation and collagen synthesis as indexes with stimulating factors serum, MCM and cytokines.

LIU Ye, QI Li-hong, WANG Shuo-feng, ZHANG Yue-fan, GUI Min, ZHANG Min, ZHANG Jun-ping. Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells[J]. Journal of Pharmaceutical Practice and Service, 2005, (6): 339-342.
Citation: LIU Ye, QI Li-hong, WANG Shuo-feng, ZHANG Yue-fan, GUI Min, ZHANG Min, ZHANG Jun-ping. Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells[J]. Journal of Pharmaceutical Practice and Service, 2005, (6): 339-342.
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