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LIU Ye, QI Li-hong, WANG Shuo-feng, ZHANG Yue-fan, GUI Min, ZHANG Min, ZHANG Jun-ping. Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells[J]. Journal of Pharmaceutical Practice and Service, 2005, (6): 339-342.
Citation:
LIU Ye, QI Li-hong, WANG Shuo-feng, ZHANG Yue-fan, GUI Min, ZHANG Min, ZHANG Jun-ping. Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells[J]. Journal of Pharmaceutical Practice and Service, 2005, (6): 339-342.
Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells
Department of Pharmacy, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350001
2.
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Received Date: 2005-09-05
Abstract
Objective To establish a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells. Methods Mouse peritoneal macrophages were primed with calcimycin 10-6mol/L for 8h then elicited by lipopolysaccharides(LPS) 100μg/L for 6h to prepare macrophage conditioned medium(MCM). Proliferative activity and collagen stimulating activity was determined by crystal violet staining assay and[3H]-proline incorporation assay using rat hepatic stellate HSC-T6 cell. Results Serum(0%-20%) and MCM(1:32-1:2) concentration-dependently enhanced HSC-T6 cell proliferation and collagen synthesis. Among IL-1, TNF, EGF, FGF and PDGF, PDGF showed the highest proliferation enhancing activity. TGFβ1 increased HSC-T6 cell collagen synthetic capacity. Conclusion It is feasible to establish an in vitro hepatic fibrosis model selecting HSC-T6 cell proliferation and collagen synthesis as indexes with stimulating factors serum, MCM and cytokines.
Abstract: Objective To establish a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells. Methods Mouse peritoneal macrophages were primed with calcimycin 10-6mol/L for 8h then elicited by lipopolysaccharides(LPS) 100μg/L for 6h to prepare macrophage conditioned medium(MCM). Proliferative activity and collagen stimulating activity was determined by crystal violet staining assay and[3H]-proline incorporation assay using rat hepatic stellate HSC-T6 cell. Results Serum(0%-20%) and MCM(1:32-1:2) concentration-dependently enhanced HSC-T6 cell proliferation and collagen synthesis. Among IL-1, TNF, EGF, FGF and PDGF, PDGF showed the highest proliferation enhancing activity. TGFβ1 increased HSC-T6 cell collagen synthetic capacity. Conclusion It is feasible to establish an in vitro hepatic fibrosis model selecting HSC-T6 cell proliferation and collagen synthesis as indexes with stimulating factors serum, MCM and cytokines.
LIU Ye, QI Li-hong, WANG Shuo-feng, ZHANG Yue-fan, GUI Min, ZHANG Min, ZHANG Jun-ping. Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells[J]. Journal of Pharmaceutical Practice and Service, 2005, (6): 339-342.
Citation:
LIU Ye, QI Li-hong, WANG Shuo-feng, ZHANG Yue-fan, GUI Min, ZHANG Min, ZHANG Jun-ping. Establishment of a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells[J]. Journal of Pharmaceutical Practice and Service, 2005, (6): 339-342.
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