2005 Vol. 23, No. 6
Display Method:
2005, (6): 326-330.
Abstract:
A new oral solid dosage form of rapidly disintegrating tablets have attracted increasing attention by its novel characteristics, such as fast disintegration, rapid absorption, high bioavailability, convient taking and so on. The dosage form brings great benefits to children and elderly people, especially those who have difficulities in swallowing. This article mainly describes the fast disintegration mechanism of orally disintegrating tablets, some preparation methods and excipients. The key quality properties are disintegrating time and dissolution. Finally, the article introduces some products that have already come into market.
A new oral solid dosage form of rapidly disintegrating tablets have attracted increasing attention by its novel characteristics, such as fast disintegration, rapid absorption, high bioavailability, convient taking and so on. The dosage form brings great benefits to children and elderly people, especially those who have difficulities in swallowing. This article mainly describes the fast disintegration mechanism of orally disintegrating tablets, some preparation methods and excipients. The key quality properties are disintegrating time and dissolution. Finally, the article introduces some products that have already come into market.
2005, (6): 339-342.
Abstract:
Objective To establish a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells. Methods Mouse peritoneal macrophages were primed with calcimycin 10-6mol/L for 8h then elicited by lipopolysaccharides(LPS) 100μg/L for 6h to prepare macrophage conditioned medium(MCM). Proliferative activity and collagen stimulating activity was determined by crystal violet staining assay and[3H]-proline incorporation assay using rat hepatic stellate HSC-T6 cell. Results Serum(0%-20%) and MCM(1:32-1:2) concentration-dependently enhanced HSC-T6 cell proliferation and collagen synthesis. Among IL-1, TNF, EGF, FGF and PDGF, PDGF showed the highest proliferation enhancing activity. TGFβ1 increased HSC-T6 cell collagen synthetic capacity. Conclusion It is feasible to establish an in vitro hepatic fibrosis model selecting HSC-T6 cell proliferation and collagen synthesis as indexes with stimulating factors serum, MCM and cytokines.
Objective To establish a liver fibrosis model in vitro by hepatic stellate HSC-T6 cells. Methods Mouse peritoneal macrophages were primed with calcimycin 10-6mol/L for 8h then elicited by lipopolysaccharides(LPS) 100μg/L for 6h to prepare macrophage conditioned medium(MCM). Proliferative activity and collagen stimulating activity was determined by crystal violet staining assay and[3H]-proline incorporation assay using rat hepatic stellate HSC-T6 cell. Results Serum(0%-20%) and MCM(1:32-1:2) concentration-dependently enhanced HSC-T6 cell proliferation and collagen synthesis. Among IL-1, TNF, EGF, FGF and PDGF, PDGF showed the highest proliferation enhancing activity. TGFβ1 increased HSC-T6 cell collagen synthetic capacity. Conclusion It is feasible to establish an in vitro hepatic fibrosis model selecting HSC-T6 cell proliferation and collagen synthesis as indexes with stimulating factors serum, MCM and cytokines.
2005, (6): 342-344.
Abstract:
Objective To study the protecting effects of extracts of the Hypericum japonicum Thunb. for the acute liver injury in rats. Methods The extracts of the Hypericum japonicum Thunb. were intraperitoneally given to every SD rat with acute liver injury induced by D-galactoasmine. It was detected that the activities of ALT and AST by using colorimetric method. Results The ethanol extract and ethyl acetate fraction of the Hypericum japonicum Thunb. significantly reduced acute liver injury induced by D-galactoasmine, by means of decreasing ALT and AST in serum. Conclusion The ethanol extract and ethyl acetate fraction of the Hypericum japonicum Thunb. have protecting effects for liver.
Objective To study the protecting effects of extracts of the Hypericum japonicum Thunb. for the acute liver injury in rats. Methods The extracts of the Hypericum japonicum Thunb. were intraperitoneally given to every SD rat with acute liver injury induced by D-galactoasmine. It was detected that the activities of ALT and AST by using colorimetric method. Results The ethanol extract and ethyl acetate fraction of the Hypericum japonicum Thunb. significantly reduced acute liver injury induced by D-galactoasmine, by means of decreasing ALT and AST in serum. Conclusion The ethanol extract and ethyl acetate fraction of the Hypericum japonicum Thunb. have protecting effects for liver.
2005, (6): 344-345.
Abstract:
Objective To investigate the difference of liver metabolism function in healthy adult female at different ages. Methods The saliva caffeine clearance(SCL) was determined by RP-HPLC method in 49 healthy adult females aged 18～34 years, 46 aged 35～50 and 18 aged 51～60 years. Results The SCL of 18～34 year group was normal. There was significant difference between 18～34 year group and others(P<0.001), significant difference was also found between 35～50 year group and 51～60 year group(P<0.001). Conclusion The SCL in female decreased with age and the liver metabolism function become lower.
Objective To investigate the difference of liver metabolism function in healthy adult female at different ages. Methods The saliva caffeine clearance(SCL) was determined by RP-HPLC method in 49 healthy adult females aged 18～34 years, 46 aged 35～50 and 18 aged 51～60 years. Results The SCL of 18～34 year group was normal. There was significant difference between 18～34 year group and others(P<0.001), significant difference was also found between 35～50 year group and 51～60 year group(P<0.001). Conclusion The SCL in female decreased with age and the liver metabolism function become lower.
2005, (6): 345-347,361.
Abstract:
Objective To observe the antidepressant activity of the active components from Hypericum sampsonii Hance. Methods The total flavonoids product from H. sampsonii were examined by Forced Swimming Test(FST) and Reserpine Reversal Test(RRT) in mice. Results Three doses(150mg/(kg·d), 300mg/(kg·d) and 600mg/(kg·d), i.g.) of the total flavonoids product(contains 68.30% total flavonoids) from H. sampsonii were examined by FST in mice, which reduced duration immobility with dose response by 5.97%, 41.85% and 45.96% respectively. In addition, the different doses of the total flavonoids(150mg/(kg·d), 300mg/(kg·d) and 600mg/(kg·d), i.g.) were examined by RRT, which induced hypothermia by low dose reserpine(5mg/kg, i.p.). In this study, the three doses of total flavonoids reversed hypothermia to some extent but with no dose response relationship. Conclusion The flavonoids compounds are the antidepressant components from H. sampsonii Hance.
Objective To observe the antidepressant activity of the active components from Hypericum sampsonii Hance. Methods The total flavonoids product from H. sampsonii were examined by Forced Swimming Test(FST) and Reserpine Reversal Test(RRT) in mice. Results Three doses(150mg/(kg·d), 300mg/(kg·d) and 600mg/(kg·d), i.g.) of the total flavonoids product(contains 68.30% total flavonoids) from H. sampsonii were examined by FST in mice, which reduced duration immobility with dose response by 5.97%, 41.85% and 45.96% respectively. In addition, the different doses of the total flavonoids(150mg/(kg·d), 300mg/(kg·d) and 600mg/(kg·d), i.g.) were examined by RRT, which induced hypothermia by low dose reserpine(5mg/kg, i.p.). In this study, the three doses of total flavonoids reversed hypothermia to some extent but with no dose response relationship. Conclusion The flavonoids compounds are the antidepressant components from H. sampsonii Hance.
2005, (6): 348-350.
Abstract:
Objective To assess the hemostatic effect and security of fibrin sealant in cardiovascular surgery. Methods 60 patients who were performed open-heart operations with incisions on aorta or right ventricle were divided randomly into test group or control group. Each group contains 30 patients. FS(test group) or NS(control group) were local used on the incisions in these patients. The hemostatic time and bleeding quantity were used as main evaluating indicators. 7 patients of test group underwent 3 months followup for the security. Results The hemostatic time(14.67±6.18s) and bleeding quantity(0.81±0.54g) of test group were obviously better than control group(250.45±37.02s and 15.39±2.69g). Conclusion Fibrin sealant has good effects of hemostasis and tissue conglutination in cardiovascular surgery.
Objective To assess the hemostatic effect and security of fibrin sealant in cardiovascular surgery. Methods 60 patients who were performed open-heart operations with incisions on aorta or right ventricle were divided randomly into test group or control group. Each group contains 30 patients. FS(test group) or NS(control group) were local used on the incisions in these patients. The hemostatic time and bleeding quantity were used as main evaluating indicators. 7 patients of test group underwent 3 months followup for the security. Results The hemostatic time(14.67±6.18s) and bleeding quantity(0.81±0.54g) of test group were obviously better than control group(250.45±37.02s and 15.39±2.69g). Conclusion Fibrin sealant has good effects of hemostasis and tissue conglutination in cardiovascular surgery.
2005, (6): 359-361.
Abstract:
Objective To investigate the preparation and quality control of compound dexamethasone liniment. Methods The compound dexamethasone liniment was prepared by taking alcohol as a solvent. The content of dexamethasone acetate in the formulation was determined by ultra-violet spectrophotometry. Results The liniment was homogenous and transparent. The calibration curve of dexamethasone acetate was linear over the concentration range of 7.5 to 17.5mg/L. The regression equation was:C=28.5299.4+0.05125, r=0.9999(n=5,P<0.01), the average recoveries of high, medium and low concentrations for dexamethasone acetate were 102.55% , 101.40% and 102.08%, respectively. The mean relative standard deviations(RSD) were 0.69%, 1.35% and 1.16%, respectively. Conclusion The formulation is simple and reasonable. The method of quality control is rapid and accurate.
Objective To investigate the preparation and quality control of compound dexamethasone liniment. Methods The compound dexamethasone liniment was prepared by taking alcohol as a solvent. The content of dexamethasone acetate in the formulation was determined by ultra-violet spectrophotometry. Results The liniment was homogenous and transparent. The calibration curve of dexamethasone acetate was linear over the concentration range of 7.5 to 17.5mg/L. The regression equation was:C=28.5299.4+0.05125, r=0.9999(n=5,P<0.01), the average recoveries of high, medium and low concentrations for dexamethasone acetate were 102.55% , 101.40% and 102.08%, respectively. The mean relative standard deviations(RSD) were 0.69%, 1.35% and 1.16%, respectively. Conclusion The formulation is simple and reasonable. The method of quality control is rapid and accurate.
2005, (6): 362-364.
Abstract:
Objective To establish a detecting method for the bacterial endotoxin in the azithromycin citrate injection. Methods The interference test was conducted with reference to Chinese Pharmacopoeia(2000ed) on BET. Results There was no interfere effect in the detection for bacterial endotoxin in the azithromycin citrate injection which was dilated one time. Conclusion The according rate is 100%, which make the pyrogen detection to the azithromycin citrate injection and experimentize contrast to the bacterial endotoxin.
Objective To establish a detecting method for the bacterial endotoxin in the azithromycin citrate injection. Methods The interference test was conducted with reference to Chinese Pharmacopoeia(2000ed) on BET. Results There was no interfere effect in the detection for bacterial endotoxin in the azithromycin citrate injection which was dilated one time. Conclusion The according rate is 100%, which make the pyrogen detection to the azithromycin citrate injection and experimentize contrast to the bacterial endotoxin.
2005, (6): 364-365.
Abstract:
Objective To establish a HPLC method for determination of astragaloside in Naomartong oral liquid. Methods Astragaloside can be determined on YWG-C18 with the mobile phase consisted of acetonitrile-0.15% H3PO4(1:2) and detected by UV at 205nm. Results The average recovery was 92.4%～97.3%. RSD was 1.5%. Conclusions The method is accurate and simple.
Objective To establish a HPLC method for determination of astragaloside in Naomartong oral liquid. Methods Astragaloside can be determined on YWG-C18 with the mobile phase consisted of acetonitrile-0.15% H3PO4(1:2) and detected by UV at 205nm. Results The average recovery was 92.4%～97.3%. RSD was 1.5%. Conclusions The method is accurate and simple.
2005, (6): 366-369.
Abstract:
Objective To establish HPLC fingerprint spectrum of commercial species of Gryllotalpa(Gryllotalpa orientalis Burmeister, G.unipina Saussure) and their false species(Teleogryllus emus). Methods Samples extracted with water by heating and refluence were analized by HPLC. HPLC mobile phase consisted of methanol-water(10:90) and detect wavelength was set at 260nm. Results Two species of Gryllotalpa and false variety had 7 common peaks, and every species had it's own characteristic peaks. Conclusion Varieties and confusion variety could be identified with differences in fingerprint by HPLC.
Objective To establish HPLC fingerprint spectrum of commercial species of Gryllotalpa(Gryllotalpa orientalis Burmeister, G.unipina Saussure) and their false species(Teleogryllus emus). Methods Samples extracted with water by heating and refluence were analized by HPLC. HPLC mobile phase consisted of methanol-water(10:90) and detect wavelength was set at 260nm. Results Two species of Gryllotalpa and false variety had 7 common peaks, and every species had it's own characteristic peaks. Conclusion Varieties and confusion variety could be identified with differences in fingerprint by HPLC.
2005, (6): 369-370.
Abstract:
Objective To establish an absorption coefficient method for determination of the dissolution of Pyrazinamide tablets. Methods The ultraviolet-spetrophotometry-absorptivity(652) was used. The absorption coefficient (E1cm1%) was 652. Results There is no significent difference between absorption coefficient method and standard method. Conclusion The method is simple, quick and accurate. It can be accepted for the quality control of pyrazinamide tablets.
Objective To establish an absorption coefficient method for determination of the dissolution of Pyrazinamide tablets. Methods The ultraviolet-spetrophotometry-absorptivity(652) was used. The absorption coefficient (E1cm1%) was 652. Results There is no significent difference between absorption coefficient method and standard method. Conclusion The method is simple, quick and accurate. It can be accepted for the quality control of pyrazinamide tablets.
2005, (6): 371-374.
Abstract:
Objective To establish the quality standard of Shengyu capsules and to investigate the acute toxicity of the capsules in mice. Methods Thin layer chromatography was used for simultaneously identification of the main components of alcohol extracts of Radix Astragali, Radix Codonopsis, Radix Paeoniae alba and Placenta Hominis in Shengyu capsules. Extractum content of Shengyu capsule was assayed by ethanol extraction method. LD50 of extractum of Shengyu capsule in mice was calculated with modified Karber's method. Results All spots were clear and concentrated on the thin layer. The results have good repeatability. The extractum content of Shengyu capsules was more than 16. 3%. LD50 and its confidence interval of Shengyu capsule in mice were 764.8 mg/kg and 682.5～847.2 mg/kg, respectively. Conclusion The method for determination the quality standard of Shengyu capsule was feasible and precise. The dosage of Shengyu capsule in clinic use was safe.
Objective To establish the quality standard of Shengyu capsules and to investigate the acute toxicity of the capsules in mice. Methods Thin layer chromatography was used for simultaneously identification of the main components of alcohol extracts of Radix Astragali, Radix Codonopsis, Radix Paeoniae alba and Placenta Hominis in Shengyu capsules. Extractum content of Shengyu capsule was assayed by ethanol extraction method. LD50 of extractum of Shengyu capsule in mice was calculated with modified Karber's method. Results All spots were clear and concentrated on the thin layer. The results have good repeatability. The extractum content of Shengyu capsules was more than 16. 3%. LD50 and its confidence interval of Shengyu capsule in mice were 764.8 mg/kg and 682.5～847.2 mg/kg, respectively. Conclusion The method for determination the quality standard of Shengyu capsule was feasible and precise. The dosage of Shengyu capsule in clinic use was safe.
Contribution System
Author Login
Review Login
Editor Login
Reader Login
News