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Volume 24 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2006  >  (6): 331-334

ZHU Zhen-yu, LIU Yu, ZHAO Bai-yun, ZHANG Hai, LI Xiang, CHAI Yi-feng. Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 331-334.
Citation: ZHU Zhen-yu, LIU Yu, ZHAO Bai-yun, ZHANG Hai, LI Xiang, CHAI Yi-feng. Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 331-334.
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Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD

  • School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • Received Date: 2006-07-14
  • Objective: To establish a method for simultaneous determination of baicalin,glycyrrbizic acid in Xiaochaihu decoction by RP-HPLC. Methods: A Agilent Zorbax SB C18（4.6mm×250mm,5m） was used with a mobile phase of acetonitrile-0.01% H3 PO4 in gradient elution,The flow rate was 1.0mL/min and the detection wavelength was set at 250nm. Results: The two constituents were separated within 50 min.The linearity was obtained over 12.66～405.0g/mL（r=0.9998）for baicalin,1.988～63.60 g/mL（r =0.9997） for glycyrrhizic acid respectively.The RSD of precision and stability of the sample was less than 1% in 48 hours.The RSD of the average recovery was less than 2%. Conclusion: The method is rapid,simple and accurate,and it can be suitable for the simulta- neous determination of baicalin,glycyrrhizic acid in Xiaochaihu decoction and the quality control of Xiaochaihu decoction.
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Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD

  • School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • Received Date: 2006-07-14

Abstract: Objective: To establish a method for simultaneous determination of baicalin,glycyrrbizic acid in Xiaochaihu decoction by RP-HPLC. Methods: A Agilent Zorbax SB C18（4.6mm×250mm,5m） was used with a mobile phase of acetonitrile-0.01% H3 PO4 in gradient elution,The flow rate was 1.0mL/min and the detection wavelength was set at 250nm. Results: The two constituents were separated within 50 min.The linearity was obtained over 12.66～405.0g/mL（r=0.9998）for baicalin,1.988～63.60 g/mL（r =0.9997） for glycyrrhizic acid respectively.The RSD of precision and stability of the sample was less than 1% in 48 hours.The RSD of the average recovery was less than 2%. Conclusion: The method is rapid,simple and accurate,and it can be suitable for the simulta- neous determination of baicalin,glycyrrhizic acid in Xiaochaihu decoction and the quality control of Xiaochaihu decoction.

ZHU Zhen-yu, LIU Yu, ZHAO Bai-yun, ZHANG Hai, LI Xiang, CHAI Yi-feng. Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 331-334.
Citation: ZHU Zhen-yu, LIU Yu, ZHAO Bai-yun, ZHANG Hai, LI Xiang, CHAI Yi-feng. Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 331-334.
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