Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD
- Received Date: 2006-07-14
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Key words:
- Xiaochaihu decoction /
- baicalin /
- glycyrrhizic acid /
- HPLC
Abstract: Objective: To establish a method for simultaneous determination of baicalin,glycyrrbizic acid in Xiaochaihu decoction by RP-HPLC. Methods: A Agilent Zorbax SB C18(4.6mm×250mm,5m) was used with a mobile phase of acetonitrile-0.01% H3 PO4 in gradient elution,The flow rate was 1.0mL/min and the detection wavelength was set at 250nm. Results: The two constituents were separated within 50 min.The linearity was obtained over 12.66~405.0g/mL(r=0.9998)for baicalin,1.988~63.60 g/mL(r =0.9997) for glycyrrhizic acid respectively.The RSD of precision and stability of the sample was less than 1% in 48 hours.The RSD of the average recovery was less than 2%. Conclusion: The method is rapid,simple and accurate,and it can be suitable for the simulta- neous determination of baicalin,glycyrrhizic acid in Xiaochaihu decoction and the quality control of Xiaochaihu decoction.
Citation: | ZHU Zhen-yu, LIU Yu, ZHAO Bai-yun, ZHANG Hai, LI Xiang, CHAI Yi-feng. Simultaneous determination of two effective components in Xiaochaihu decoction by HPLC/DAD[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 331-334. |