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FU Xiang, FAN Shang-tan, LI Jin-lan, ZHANG Yi-fan, XIE Bao-ying. Determination of content of hyoscyamine in compound belladonna mixture by acid dye colorimetry[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 334-336.
Citation: FU Xiang, FAN Shang-tan, LI Jin-lan, ZHANG Yi-fan, XIE Bao-ying. Determination of content of hyoscyamine in compound belladonna mixture by acid dye colorimetry[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 334-336.

Determination of content of hyoscyamine in compound belladonna mixture by acid dye colorimetry

  • Received Date: 2006-01-13
  • Objective: To determine hyoscyamine in compound belladonna mixture. Methods: Acid dye colorimetry was used in pH 6.6 with the detection wavelength at(589±1)nm. Results: The linear ranges of the standard curve was 1.2~7.2μg/mL(r= 0.9991).The average recovery of the assay was 100.02%(RSD=0.69%,n=4). Conclusions: This method is accurate,convenient and sensitive for the quality control of hyoscyamine in compound belladonna mixture.The key of this method is exactly control of pH in order to avoid the disturbance.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of content of hyoscyamine in compound belladonna mixture by acid dye colorimetry

Abstract: Objective: To determine hyoscyamine in compound belladonna mixture. Methods: Acid dye colorimetry was used in pH 6.6 with the detection wavelength at(589±1)nm. Results: The linear ranges of the standard curve was 1.2~7.2μg/mL(r= 0.9991).The average recovery of the assay was 100.02%(RSD=0.69%,n=4). Conclusions: This method is accurate,convenient and sensitive for the quality control of hyoscyamine in compound belladonna mixture.The key of this method is exactly control of pH in order to avoid the disturbance.

FU Xiang, FAN Shang-tan, LI Jin-lan, ZHANG Yi-fan, XIE Bao-ying. Determination of content of hyoscyamine in compound belladonna mixture by acid dye colorimetry[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 334-336.
Citation: FU Xiang, FAN Shang-tan, LI Jin-lan, ZHANG Yi-fan, XIE Bao-ying. Determination of content of hyoscyamine in compound belladonna mixture by acid dye colorimetry[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 334-336.

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