Quantitative determination of luteolin in Pyrrosia lingua by HPLC
- Received Date: 2005-07-03
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Key words:
- Pyrrosia lingua /
- luteolin /
- HPLC
Abstract: Objective: To determine the content of luteolin in Pyrrosia lingua by HPLC. Methods: The HPLC method was used to deter- mine the content of luteolin from Pyrrosia lingua.The analysis was carried out on Shimadzu C18 colunm(150mm×4.6mm,5μm).The mobile phase was MeOH-0.2% H3 PO4(50:50).Flow-rate was 1.0mL/min.Wave-length was 254nm.Temperature was room temperature. Results: The method was simple and had a good linear relationship.The liner range of luteolin is 0.40~2.00μg and gave a correlation (r)of 0.999 6.The recovery,was 95.85%,RSD 0.27%. Conclusion: The method was easy to determine the content of luteolin from Pyrrosia lingua.
Citation: | ZHANG Ya-ning, WU Bo, YANG Ji-gang, XU Xiu-ying, ZHENG Yi-min. Quantitative determination of luteolin in Pyrrosia lingua by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 359-361. |