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Volume 25 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2007  >  (6): 379-381

KONG Ling-yuan, MA Jun. HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 379-381.
Citation: KONG Ling-yuan, MA Jun. HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 379-381.
shu

HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study

  • 1.

    Department of Pharmacy,No.451 Hospital of PLA,Xi'an 710000,China;Department of Pharmacy,General Hospital of Lanzhou Command of PLA,Lanzhou 730050,China

  • 2.

    Department of Pharmacy,No.451 Hospital of PLA,Xi'an 710000,China;Department of Pharmacy,General Hospital of Lanzhou Command of PLA,Lanzhou 730050,China

  • Received Date: 2006-12-25
  • Objective :To establish a simple,rapid,sensitive column-switching HPLC method for the analysis of the 10-hydroxycamptothecin（HCPT） in human serum. Methods : A precolumn containing restricted access media（RAM） was used for the sample clean-up and trace enrichment and was combined with a C18 column for the final separation.The HCPT was monitored with fluorescence detector,excitation and emission wavelengths being 385 and 539 nm,respectively. Results : The analytical time was 8 minutes.There was a linear response range of 1-1 000 ng/mL with correlation coefficient of 0.998 while the limit of detection was 0.1 ng/mL.The intraday and interday variations were less than 5%. Conclusion : This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model were calculated.
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HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study

  • 1. Department of Pharmacy,No.451 Hospital of PLA,Xi'an 710000,China;Department of Pharmacy,General Hospital of Lanzhou Command of PLA,Lanzhou 730050,China
  • 2. Department of Pharmacy,No.451 Hospital of PLA,Xi'an 710000,China;Department of Pharmacy,General Hospital of Lanzhou Command of PLA,Lanzhou 730050,China
  • Received Date: 2006-12-25

Abstract: Objective :To establish a simple,rapid,sensitive column-switching HPLC method for the analysis of the 10-hydroxycamptothecin（HCPT） in human serum. Methods : A precolumn containing restricted access media（RAM） was used for the sample clean-up and trace enrichment and was combined with a C18 column for the final separation.The HCPT was monitored with fluorescence detector,excitation and emission wavelengths being 385 and 539 nm,respectively. Results : The analytical time was 8 minutes.There was a linear response range of 1-1 000 ng/mL with correlation coefficient of 0.998 while the limit of detection was 0.1 ng/mL.The intraday and interday variations were less than 5%. Conclusion : This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model were calculated.

KONG Ling-yuan, MA Jun. HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 379-381.
Citation: KONG Ling-yuan, MA Jun. HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 379-381.
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