Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 25 Issue 6
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2007  >  (6): 379-381

KONG Ling-yuan, MA Jun. HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 379-381.
Citation: KONG Ling-yuan, MA Jun. HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 379-381.
shu

HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study

  • 1.

    Department of Pharmacy,No.451 Hospital of PLA,Xi'an 710000,China;Department of Pharmacy,General Hospital of Lanzhou Command of PLA,Lanzhou 730050,China

  • 2.

    Department of Pharmacy,No.451 Hospital of PLA,Xi'an 710000,China;Department of Pharmacy,General Hospital of Lanzhou Command of PLA,Lanzhou 730050,China

  • Received Date: 2006-12-25
  • Objective :To establish a simple,rapid,sensitive column-switching HPLC method for the analysis of the 10-hydroxycamptothecin（HCPT） in human serum. Methods : A precolumn containing restricted access media（RAM） was used for the sample clean-up and trace enrichment and was combined with a C18 column for the final separation.The HCPT was monitored with fluorescence detector,excitation and emission wavelengths being 385 and 539 nm,respectively. Results : The analytical time was 8 minutes.There was a linear response range of 1-1 000 ng/mL with correlation coefficient of 0.998 while the limit of detection was 0.1 ng/mL.The intraday and interday variations were less than 5%. Conclusion : This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model were calculated.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2477) PDF downloads(45) Cited by()

Related
Proportional views

HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study

  • 1. Department of Pharmacy,No.451 Hospital of PLA,Xi'an 710000,China;Department of Pharmacy,General Hospital of Lanzhou Command of PLA,Lanzhou 730050,China
  • 2. Department of Pharmacy,No.451 Hospital of PLA,Xi'an 710000,China;Department of Pharmacy,General Hospital of Lanzhou Command of PLA,Lanzhou 730050,China
  • Received Date: 2006-12-25

Abstract: Objective :To establish a simple,rapid,sensitive column-switching HPLC method for the analysis of the 10-hydroxycamptothecin（HCPT） in human serum. Methods : A precolumn containing restricted access media（RAM） was used for the sample clean-up and trace enrichment and was combined with a C18 column for the final separation.The HCPT was monitored with fluorescence detector,excitation and emission wavelengths being 385 and 539 nm,respectively. Results : The analytical time was 8 minutes.There was a linear response range of 1-1 000 ng/mL with correlation coefficient of 0.998 while the limit of detection was 0.1 ng/mL.The intraday and interday variations were less than 5%. Conclusion : This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model were calculated.

KONG Ling-yuan, MA Jun. HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 379-381.
Citation: KONG Ling-yuan, MA Jun. HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 379-381.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return