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2007 Vol. 25, No. 6

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Current advances in the study on rutaecarpine
YU Qi, GUO Cheng, CHENG Ze-neng
2007, (6): 353-357.
Abstract(2718)
Abstract:
The rutaecarpine is an effective component from Evodia rutaecarpa(Juss.) Benth.Current advances in the study of rutaecarpine is reviewed,including synthesize,effects on metabolizing enzyme,metabolic mechanism, pharmacological activity,etc.
2007, (6): 358-360.
Abstract(2125)
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2007, (6): 361-363.
Abstract(1946)
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2007, (6): 364-366,392.
Abstract(2182)
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2007, (6): 367-368,385.
Abstract(2087)
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Studies on the application of the hyperlipemia model of golden hamster and Wistar rat
LI Lin-peng, SUN Jun-wen, CAO Yong-bing, REN Tian-shu, JIANG Yuan-ying, SUN De-hao
2007, (6): 369-371.
Abstract(2750)
Abstract:
Objective :To compare the effect of simvastatin in the hyperlipemia model of the golden hamster and the Wistar rat,discuss the reasonable application of the two animal hyperlipemia models. Methods :Thirty male golden hamsters weighing 100-130 g and thirty male Wistar rat weighing 200-300 g were respectively randomized into 3 groups with 10 in each,named as the normal group,model group and simvastatin group.After the models of hyperlipemia were established,the simvastatin groups were treated with the dosage of 10mg/(kg·d) simvastatin.Observe the effect of simvastatin on lipid level in the 14th and 28th day.Analysed the effect of simvastatin in the two hyperlipemia models. Results :Simvastatin decreased the lipid level of the two hyperlipemia model.The effect in Wistar rat is better than in golden hamster. Conclusion : Wistar rat is more susceptible to simvastatin than golden hamster.The hyperlipemia model of Wistar rat is adapt to the application of the selection of cholesterol-lowering drugs.The hyperlipemia model of golden hamster is suit to the before clinical research of the novel cholesterol-lowering drugs.
Study on the structural features of colchicine-site inhibitors
LI Yao-wu, ZHOU You-jun, ZHU Ju, ZHENG Can-hui, CHEN Jun, TANG Hui, LI Ya-nan
2007, (6): 372-375.
Abstract(2462)
Abstract:
Objective :To determine the structural features of a set of diverse colchicines-site inhibitors. Methods :The features were revealed by matching the binding conformations generated from docking studies of eight inhibitors,and combined with the available SAR results. Results : The feature is represented as two hydrophobic groups(A and B) and a bridge linked between them,but A and B should be in the same side of the bridge.The volume size and the existence of hydrogen-bond acceptor of group A may have some influence on binding.Group B should be a hydrophobic planar ring,and polar functional group is advantageous.The bridge part could accept 1-4 atoms,but the Sp2 hybrid is welcome. Conclusion :this result may provide useful insights for the design of novel Colchicine-site inhibitors.
Studies on hydrolysis of cefotaxime by broad and extended-spectrum-β-lactamase and inhibitory actions of tazobactam and sulbactam
ZHANG Xiang, LEI Jun, YUAN Bin, LIU Gang, ZHAO Ting-kun, LING Bao-dong
2007, (6): 376-378.
Abstract(2116)
Abstract:
Objective :To observe the stability of cefotaxime(CTX) to broad-and extended-spectrum β-lactamase,and to compare inhibiting-βlactamase activities of tazobactam and sulbactam. Methods : 60 strains of Gram-negative bacilli from Affiliated Hospital of North Sichuan Medical College were dealed with ultrasonic wave and their β-lactamases were extracted and these enzymes were detected by three-dimensional test.β-lactamase activitiy and hydrolysis of CTX were quantitated spectrophotometrically and calculated. Results :In these β-lactamases of 60 strains of Gram-negitive bacilli,the number of extended-spectrum β-lactamases and broad-spectrum β-lactamases were 31 and 29,respectively,and their medians of enzyme activities were 3 094 U and 528 U(P<0.05).The medians of β-lactamases activities of Escherichia coli,Enterobacter cloacae and Pseudomonas aeruginosa were 2 656 U,1 185 U and 165.5 U,respectively.The median of hydrolysis rates(HR) of CTX by ESBLs and BSBLs were 20.10% and 3.15%,respectively(P<0.05).The combination of CTX/TAZ and CTX/SBT(the ratio=2-1) could make the HR drop significantly(P<0.05),but the effects of TAZ and SBT were of no differences(P>0.05). Conclusion :The hydrolysis activities of ESBLs on CTX was higher than that of BSBLs.TAZ and SBT could effectively inhibit hydrolysis of CTX by β-lactamases,but these two inhibitors had little difference in their potency.
HPLC analysis of 10-hydroxycamptothecin and its application to clinical pharmacokinetic study
KONG Ling-yuan, MA Jun
2007, (6): 379-381.
Abstract(2410)
Abstract:
Objective :To establish a simple,rapid,sensitive column-switching HPLC method for the analysis of the 10-hydroxycamptothecin(HCPT) in human serum. Methods : A precolumn containing restricted access media(RAM) was used for the sample clean-up and trace enrichment and was combined with a C18 column for the final separation.The HCPT was monitored with fluorescence detector,excitation and emission wavelengths being 385 and 539 nm,respectively. Results : The analytical time was 8 minutes.There was a linear response range of 1-1 000 ng/mL with correlation coefficient of 0.998 while the limit of detection was 0.1 ng/mL.The intraday and interday variations were less than 5%. Conclusion : This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model were calculated.
Analysis of the lignans components in decoction granule of Schisandra chinensis
ZHANG Lan, YANG Shui-xin
2007, (6): 382-385.
Abstract(2262)
Abstract:
Objective :To establish a method to determine the active components of lignans(schisandrin,schisandtherin A,deoxyschizandrin and schisandrin B) in decoction granule of Schisandra chinensis.To find and anylyse the difference of components of different factories. Methods :RP-HPLC method was used.The column was Hypersil ODS C18(4.6 mm×250 mm,10 μm) with the mobile phase of acetonititrile(A) and H2O(B),the flow rate was 1.0 mL/min,the column temperature was set at 25 ℃ and the detection wavelength was set at 254 nm. Results : The content of schisandrin was from 0 to 1.292 3mg/package,schisandtherin A was from 0 to 0.560 8 mg/package,deoxyschizandrin was from 0 to 0.168 8mg/package.Schisandrin B was not detected. Conclusion : The content of schisandrin,schisandtherin A and deoxyschizandrin were obviously different in decoction granule of Schisandra chinensis produced by different factories.
Effect analysis of the concentration of Liuwei Dihuang polysaccharides by the membrane separation technique and the traditional method
ZHONG Lei, ZHAO Han-chen, YAN Hui, WANG Rui, WANG Su-hui
2007, (6): 386-387,390.
Abstract(2712)
Abstract:
Objective :To compare the effect between the membrane separation technique and the traditional method being used to concentrate polysaccharides from Liuwei Dihuang formula. Methods : The extract liquid of Liuwei Dihuang formula was divided into several levels and segments using nanofiltration and ultrafiltration of 10 000Mr.The polysaccharides in every level's extracted liquid were quantitate by sulfuric acid-anthrone colorimetric method at detecting wavelength 625 nm. Results : The polysaccharides in the production made by membrane separation technique were 6.5 times as much as that in the production made by the traditional method. Conclusion : The membrane separation technique was superior in concentration polysaccharides compared with the traditional method.
Detection of gibenclamide and phenformin in Jiangtang capsule by liquid chromatography-electrospray Ionization tandem mass spectrometry
ZHENG Cheng, XIANG Zhi-min
2007, (6): 388-390.
Abstract(2358)
Abstract:
Objective :To establish a liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI-MS-MS) for the identification of glibenclamide and phenformin illegally added in Jiangtang Capsule. Methods :The detection was performed on Altima C18 column with a mobile phase of acetonitrile-formic acid solution(65-35).The above compounds were identified by LC-MS-MS. Results :Glibenclamide and phenformin were detected illegally added in one sample of Jiangtang capsule. Conclusion :This is an effective method with characteristic and sensitivity for analysis of chemical drugs added in TCM.
Content determination of aconitine in Shangke liniment by HPLC
ZHANG Mei-ling
2007, (6): 391-391.
Abstract(2684)
Abstract:
Objective :To set up an HPLC method for the content determination of aconitine in shangke liniment. Methods :Chromatography was performed on a MN-Nucleosil 7C6H5 stainless steel column(25 cm×4.0 mm).The mobile phase was a mixture of methanol-water-chloroform-triethylamine(70-30-1-0.2),with a flow rate of 1.5 mL/min.The detection wavelength was 235 nm. Results :The calibration curve was linear over the range of 0.050-0.225 μg,the average rate of recovery was 95.42 %,RSD=1.62 %(n=5). Conclusion :The method proved to be simple,rapid,reliable and well reproducible in the content determination of aconitine in shangke liniment.It may be used as a method of quality control of the preparation.
Drug utilization analysis of oral hypoglycemic agents in our hospital during the period of 20032005
XIN Hai-li
2007, (6): 393-395.
Abstract(1757)
Abstract:
Objective :To assess the drug utilization and tendency of progress of oral hypoglycemic agents in our hospital and to provide references for rational administration clinically. Methods :DDDs,sales quantities,sales expenses and the ratio of sales expenses sequencing to that of DDDs in our hospital during the period of 2003-2005 were analyzed statistically with the method of DDDs analysis. Results :The top two antidiabetic drugs according to DDDs were melbine (dimethyl diguanide) and gliclazide three successive years.The DDDs of acarbose increased dramatically.Which blood glucose regulatsry drugs ranked lower,the ratio of sales expenses sequencing to that of DDDs of glipizide and gliclazide controlled release pellets was equal or about,whereas that of rosiglitazone,acarbose (glucobay) and glimepiride was less than 1. Conclusion :The study shows that the drug utilization of oral hypoglycemic agents in our hospital is basically rational and is in accordance with the trend of advancement and drug therapeutic strategies of diabetes mellitus.
Effects of Qishenfukang capsule on improvement of physical and intellectual function on rats
LI Xin-tian, YANG Lai-qi, QIU Cai-rong, CHEN Chong-shang
2007, (6): 396-397.
Abstract(2351)
Abstract:
Objective :To investigated the effects of Qishenfukang capsule improving the physical and intellective function in rats,in order to provide laboratory evidence for clinical usage. Methods :The rats were divided into four groups.Qishenfukang capsule(0.3 g,0.6 g,1.2 g/kg)and placebo groups,respectively.Then their body weight,swimming ability and memory were tested. Results :Qishenfukang capsule could increase those rats' weight,prolong their swimming time and improve their memory obviously. Conclusion :Qishenfukang capsule could promote rats' strength and intellectual function.
2007, (6): 398-398.
Abstract(1900)
Abstract:
Clinical curative effect analysis of gefitinib for treat NSCLC
JIA Guo-qiang, YANG Ya-qing
2007, (6): 399-400.
Abstract(2304)
Abstract:
Objective :To analyze clinical curative effect of Gefitinib to treat NSCLC(non-small cell lung cancer). Methods :40 cases in-patient with NSCLC in Hospital of PLA General Hospital during Juan 2005 to Dec 2006,were administrated Gefitinib 250 mg daily orally,and investigated for it's curative effect. Results :The total effective rate was 35.0%,CR(complete remission)1 case,PR(partial remission) 13 cases,the disease control rate was 82.5%(CR was 1 case,PR was 13 cases,basio-stabilization was 19 cases).There were 29 male patients and 11 females,and effective rate were 55.2% and 36.4% respectively(P<0.05).The main adverse reaction were nausea and vomiting(37%),anorexy(25%),and exanthem(18%). Conclusion : Gefitinib has a better curative effect to treat NSCLC,and the adverse effect is gently,it can improve majority patient quality of life and survival.
2007, (6): 401-403.
Abstract(1967)
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Study on the pharmacokinetics of caffeine in male at different ages
LI Qun-an
2007, (6): 403-404.
Abstract(2258)
Abstract:
Objective :To obtain the pharmacokinetics parameters of caffeine in the elderly,presenile,pastadolescenal and young male. Methods :Saliva caffeine(CAF) concentration in 16 old male(aged 67~81 years),20 presenile male(aged 51~60 years),46 pastadolescenal male(aged 35~50 years) and 95 young male(aged 18~34 years) was determined by RP-HPLC method,and according to the saliva concentration time curves,the pharmacokinetic parameters were calculated. Results : The saliva CAF1/2 in the elderly group was significantly increased to those in the other group(P<0.000 5,P<0.000 1,P<0.01),the saliva CAF clearanc was significantly decreased(P<0.000 2,P<0.002,P<0.01) and apparent volume of distribution(Vd) was not different significantly(P>0.05). Conclusion : The saliva CAF1/2 and clearanc in the elderly male were significantly increased and decreased respectively,Vd was not different significantly.
2007, (6): 405-406.
Abstract(1678)
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2007, (6): 407-409.
Abstract(1532)
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2007, (6): 410-412.
Abstract(1812)
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2007, (6): 412-414.
Abstract(1922)
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2007, (6): 415-417.
Abstract(1464)
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2007, (6): 417-418.
Abstract(1582)
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2007, (6): 419-420.
Abstract(1759)
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2007, (6): 420-420.
Abstract(2829)
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2007, (6): 421-422,427.
Abstract(1606)
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2007, (6): 423-424.
Abstract(1830)
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2007, (6): 425-427.
Abstract(1791)
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2007, (6): 427-427.
Abstract(2092)
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2007, (6): 428-431.
Abstract(1645)
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