Detection of gibenclamide and phenformin in Jiangtang capsule by liquid chromatography-electrospray Ionization tandem mass spectrometry

Key words: 
• traditional Chinese medicine(TCM) /
• glibenclamide /
• phenformin hydrochloride /
• LC-MS-MS

Abstract: Objective :To establish a liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI-MS-MS) for the identification of glibenclamide and phenformin illegally added in Jiangtang Capsule. Methods :The detection was performed on Altima C₁₈ column with a mobile phase of acetonitrile-formic acid solution(65-35).The above compounds were identified by LC-MS-MS. Results :Glibenclamide and phenformin were detected illegally added in one sample of Jiangtang capsule. Conclusion :This is an effective method with characteristic and sensitivity for analysis of chemical drugs added in TCM.