Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction
- Received Date: 2006-07-14
- Rev Recd Date: 2006-09-11
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Key words:
- compound Er-xian decoction /
- berberine hydrochloride /
- total alkaloids /
- HPLC /
- visible spectrometry
Abstract: Objective: To establish a quantitative analysis method for berberine hydrochloride and total alkaloids in compound Er-xian decoction. Methods: Berberine hydrochloride were determined by HPLC. Using Hypersil-BDS C18 (4.6 x 200 mm) column, methanol-0.02 mol/L sodium dihydrogen phosphate-0.1 mol/L triethylamine (40-60-2) as the mobile phase. The detection wavelength was 345 nm. Total alkaloids were determined at 420 nm by visible spectrometry. Results: The linearity range of berberine hydrochloride was 0.51~10.10 μg/mL, r=0.999 9, and average recovery was 100.07%,RSD 0.4%. The linearity range of total alkaloids was 8.0~24.0 μg/mL, r=0.999 8, and average recovery was 100.46%, RSD 0.58%. Conclusions: The method is simple, quick and accurate.It can be used to control the quality of the decoction preparation.
Citation: | NIAN Hua, XU Ling-ling, MA Ming-hua, ZHENG Han-chen, ZHANG Qiao-yan. Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 38-40,60. |