Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 26 Issue 1
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2008  >  (1): 38-40,60

NIAN Hua, XU Ling-ling, MA Ming-hua, ZHENG Han-chen, ZHANG Qiao-yan. Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 38-40,60.
Citation: NIAN Hua, XU Ling-ling, MA Ming-hua, ZHENG Han-chen, ZHANG Qiao-yan. Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 38-40,60.
shu

Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction

  • 1.

    Department of Pharmacy,Yueyang Hospital of Chinese Integrative Medicine,Shanghai 200437, China

  • 2.

    Shanghai Xinxing Medicine Co.Ltd. Shanghai 200135, China

  • 3.

    School of Pharmacy,Second Military Medical University,Shanghai 200433, China

  • Received Date: 2006-07-14
  • Rev Recd Date: 2006-09-11
  • Objective: To establish a quantitative analysis method for berberine hydrochloride and total alkaloids in compound Er-xian decoction. Methods: Berberine hydrochloride were determined by HPLC. Using Hypersil-BDS C18 (4.6 x 200 mm) column, methanol-0.02 mol/L sodium dihydrogen phosphate-0.1 mol/L triethylamine (40-60-2) as the mobile phase. The detection wavelength was 345 nm. Total alkaloids were determined at 420 nm by visible spectrometry. Results: The linearity range of berberine hydrochloride was 0.51～10.10 μg/mL, r=0.999 9, and average recovery was 100.07%,RSD 0.4%. The linearity range of total alkaloids was 8.0～24.0 μg/mL, r=0.999 8, and average recovery was 100.46%, RSD 0.58%. Conclusions: The method is simple, quick and accurate.It can be used to control the quality of the decoction preparation.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2548) PDF downloads(997) Cited by()

Related
Proportional views

Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction

  • 1. Department of Pharmacy,Yueyang Hospital of Chinese Integrative Medicine,Shanghai 200437, China
  • 2. Shanghai Xinxing Medicine Co.Ltd. Shanghai 200135, China
  • 3. School of Pharmacy,Second Military Medical University,Shanghai 200433, China
  • Received Date: 2006-07-14
  • Rev Recd Date: 2006-09-11

Abstract: Objective: To establish a quantitative analysis method for berberine hydrochloride and total alkaloids in compound Er-xian decoction. Methods: Berberine hydrochloride were determined by HPLC. Using Hypersil-BDS C18 (4.6 x 200 mm) column, methanol-0.02 mol/L sodium dihydrogen phosphate-0.1 mol/L triethylamine (40-60-2) as the mobile phase. The detection wavelength was 345 nm. Total alkaloids were determined at 420 nm by visible spectrometry. Results: The linearity range of berberine hydrochloride was 0.51～10.10 μg/mL, r=0.999 9, and average recovery was 100.07%,RSD 0.4%. The linearity range of total alkaloids was 8.0～24.0 μg/mL, r=0.999 8, and average recovery was 100.46%, RSD 0.58%. Conclusions: The method is simple, quick and accurate.It can be used to control the quality of the decoction preparation.

NIAN Hua, XU Ling-ling, MA Ming-hua, ZHENG Han-chen, ZHANG Qiao-yan. Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 38-40,60.
Citation: NIAN Hua, XU Ling-ling, MA Ming-hua, ZHENG Han-chen, ZHANG Qiao-yan. Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 38-40,60.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return