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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2008  >  (1): 38-40,60

NIAN Hua, XU Ling-ling, MA Ming-hua, ZHENG Han-chen, ZHANG Qiao-yan. Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 38-40,60.
Citation: NIAN Hua, XU Ling-ling, MA Ming-hua, ZHENG Han-chen, ZHANG Qiao-yan. Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 38-40,60.
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Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction

  • 1.

    Department of Pharmacy,Yueyang Hospital of Chinese Integrative Medicine,Shanghai 200437, China

  • 2.

    Shanghai Xinxing Medicine Co.Ltd. Shanghai 200135, China

  • 3.

    School of Pharmacy,Second Military Medical University,Shanghai 200433, China

  • Received Date: 2006-07-14
  • Rev Recd Date: 2006-09-11
  • Objective: To establish a quantitative analysis method for berberine hydrochloride and total alkaloids in compound Er-xian decoction. Methods: Berberine hydrochloride were determined by HPLC. Using Hypersil-BDS C18 (4.6 x 200 mm) column, methanol-0.02 mol/L sodium dihydrogen phosphate-0.1 mol/L triethylamine (40-60-2) as the mobile phase. The detection wavelength was 345 nm. Total alkaloids were determined at 420 nm by visible spectrometry. Results: The linearity range of berberine hydrochloride was 0.51～10.10 μg/mL, r=0.999 9, and average recovery was 100.07%,RSD 0.4%. The linearity range of total alkaloids was 8.0～24.0 μg/mL, r=0.999 8, and average recovery was 100.46%, RSD 0.58%. Conclusions: The method is simple, quick and accurate.It can be used to control the quality of the decoction preparation.
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  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction

  • 1. Department of Pharmacy,Yueyang Hospital of Chinese Integrative Medicine,Shanghai 200437, China
  • 2. Shanghai Xinxing Medicine Co.Ltd. Shanghai 200135, China
  • 3. School of Pharmacy,Second Military Medical University,Shanghai 200433, China
  • Received Date: 2006-07-14
  • Rev Recd Date: 2006-09-11

Abstract: Objective: To establish a quantitative analysis method for berberine hydrochloride and total alkaloids in compound Er-xian decoction. Methods: Berberine hydrochloride were determined by HPLC. Using Hypersil-BDS C18 (4.6 x 200 mm) column, methanol-0.02 mol/L sodium dihydrogen phosphate-0.1 mol/L triethylamine (40-60-2) as the mobile phase. The detection wavelength was 345 nm. Total alkaloids were determined at 420 nm by visible spectrometry. Results: The linearity range of berberine hydrochloride was 0.51～10.10 μg/mL, r=0.999 9, and average recovery was 100.07%,RSD 0.4%. The linearity range of total alkaloids was 8.0～24.0 μg/mL, r=0.999 8, and average recovery was 100.46%, RSD 0.58%. Conclusions: The method is simple, quick and accurate.It can be used to control the quality of the decoction preparation.

NIAN Hua, XU Ling-ling, MA Ming-hua, ZHENG Han-chen, ZHANG Qiao-yan. Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 38-40,60.
Citation: NIAN Hua, XU Ling-ling, MA Ming-hua, ZHENG Han-chen, ZHANG Qiao-yan. Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction[J]. Journal of Pharmaceutical Practice and Service, 2008, (1): 38-40,60.
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