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LIU Hou-jia, LI Tie-jun, QIU Yan, RUI Rao-cheng, WEI Li-xin, WU Meng-chao, ZHANG Li. Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 94-98.
Citation: LIU Hou-jia, LI Tie-jun, QIU Yan, RUI Rao-cheng, WEI Li-xin, WU Meng-chao, ZHANG Li. Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 94-98.

Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin

  • Received Date: 2008-08-28
  • Objective : To study the protective effects of imperatorin(IMP) against human umbilical vein endothelial cells(HUVEC) damage and expression of vascular endothelial growth factor(VEGF) induced by lysophosphatidvlcholine(LPC). Methods : Cell iniury were determined by MTT assay.Vascular endothelial growth factor protein production from HUVEC was determined by enzyme-linked immunosorbent assay(ELISA).VEGF mRNA expression was examined by RT-PCR and realtime PCR. Results :Imperatorin prevented LPC-induced injury in cultured HUVEC in a concentration-dependent manner.LPC induced expression of VEGF protein and VEGF mRNA on HUVEC.Imperatorin could inhibit the expression of VEGF protein an d VEGF mRNA in co-cultured with LPC. Conclusions :LPC could induce cell damage and a strong expression of VEGF on HUVEC.Imperatorin could protect HUVEC against the LPC-induced damage and downregulate VEGF protein and VEGF mRNA expression in cultured HUVEC.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin

Abstract: Objective : To study the protective effects of imperatorin(IMP) against human umbilical vein endothelial cells(HUVEC) damage and expression of vascular endothelial growth factor(VEGF) induced by lysophosphatidvlcholine(LPC). Methods : Cell iniury were determined by MTT assay.Vascular endothelial growth factor protein production from HUVEC was determined by enzyme-linked immunosorbent assay(ELISA).VEGF mRNA expression was examined by RT-PCR and realtime PCR. Results :Imperatorin prevented LPC-induced injury in cultured HUVEC in a concentration-dependent manner.LPC induced expression of VEGF protein and VEGF mRNA on HUVEC.Imperatorin could inhibit the expression of VEGF protein an d VEGF mRNA in co-cultured with LPC. Conclusions :LPC could induce cell damage and a strong expression of VEGF on HUVEC.Imperatorin could protect HUVEC against the LPC-induced damage and downregulate VEGF protein and VEGF mRNA expression in cultured HUVEC.

LIU Hou-jia, LI Tie-jun, QIU Yan, RUI Rao-cheng, WEI Li-xin, WU Meng-chao, ZHANG Li. Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 94-98.
Citation: LIU Hou-jia, LI Tie-jun, QIU Yan, RUI Rao-cheng, WEI Li-xin, WU Meng-chao, ZHANG Li. Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 94-98.

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