2009 Vol. 27, No. 2
Display Method:
2009, 27(2): 90-93.
Abstract:
Objective : To investigate the action of Qizhiweitong granule(QZ) on gastrointestinal function and analgesic effect in mice. Methods : Dextran blue-2000 was used as a marker,both its residue left in the stomach and propulsion velocity in the intestine were determined for the evaluation of gastrointestinal function;with acetic acid induced mice writhing response model,analgesic effect of QZ was determined. Results : QZ 1.25 g/kg,2.50 g/kg,5.00 g/kg improved the inhibition of gastric empty and intestinal propulsion induced by both atropine and dopamine.No changes were observed in morphine-induced gastrointestinal disorder mice model.All the three doses exhibited analgesic effect on acetic acid induced mice writhing response model. Conclusion :These results suggest QZ maybe used to improve gastrointestinal function and possessed analgesic effects.
Objective : To investigate the action of Qizhiweitong granule(QZ) on gastrointestinal function and analgesic effect in mice. Methods : Dextran blue-2000 was used as a marker,both its residue left in the stomach and propulsion velocity in the intestine were determined for the evaluation of gastrointestinal function;with acetic acid induced mice writhing response model,analgesic effect of QZ was determined. Results : QZ 1.25 g/kg,2.50 g/kg,5.00 g/kg improved the inhibition of gastric empty and intestinal propulsion induced by both atropine and dopamine.No changes were observed in morphine-induced gastrointestinal disorder mice model.All the three doses exhibited analgesic effect on acetic acid induced mice writhing response model. Conclusion :These results suggest QZ maybe used to improve gastrointestinal function and possessed analgesic effects.
2009, 27(2): 94-98.
Abstract:
Objective : To study the protective effects of imperatorin(IMP) against human umbilical vein endothelial cells(HUVEC) damage and expression of vascular endothelial growth factor(VEGF) induced by lysophosphatidvlcholine(LPC). Methods : Cell iniury were determined by MTT assay.Vascular endothelial growth factor protein production from HUVEC was determined by enzyme-linked immunosorbent assay(ELISA).VEGF mRNA expression was examined by RT-PCR and realtime PCR. Results :Imperatorin prevented LPC-induced injury in cultured HUVEC in a concentration-dependent manner.LPC induced expression of VEGF protein and VEGF mRNA on HUVEC.Imperatorin could inhibit the expression of VEGF protein an d VEGF mRNA in co-cultured with LPC. Conclusions :LPC could induce cell damage and a strong expression of VEGF on HUVEC.Imperatorin could protect HUVEC against the LPC-induced damage and downregulate VEGF protein and VEGF mRNA expression in cultured HUVEC.
Objective : To study the protective effects of imperatorin(IMP) against human umbilical vein endothelial cells(HUVEC) damage and expression of vascular endothelial growth factor(VEGF) induced by lysophosphatidvlcholine(LPC). Methods : Cell iniury were determined by MTT assay.Vascular endothelial growth factor protein production from HUVEC was determined by enzyme-linked immunosorbent assay(ELISA).VEGF mRNA expression was examined by RT-PCR and realtime PCR. Results :Imperatorin prevented LPC-induced injury in cultured HUVEC in a concentration-dependent manner.LPC induced expression of VEGF protein and VEGF mRNA on HUVEC.Imperatorin could inhibit the expression of VEGF protein an d VEGF mRNA in co-cultured with LPC. Conclusions :LPC could induce cell damage and a strong expression of VEGF on HUVEC.Imperatorin could protect HUVEC against the LPC-induced damage and downregulate VEGF protein and VEGF mRNA expression in cultured HUVEC.
2009, 27(2): 99-100,103.
Abstract:
Objective : To study the chemical constituents of marine starfish Asterina pectinifera. Methods : The constituents were separated and purified by column chromatography with silica gel and sephadex LH-20.Their structures were identified by spectrum and physical data. Results :Seven compounds were identified as : 3-(octadecyloxy)-1,2-Propanediol(1),5α-Cholest-7-en-3β-ol(2),asterosapoin P1(3),5α-cholestane-3β,6α,7α,8,15α,16β,26-heptol(4),5α-cholestane-3β,4β,6α,7α,8,15β,16β,26-octol(5),tryptophan(6),phenylalanine(7). Conclusions :Compounds 1 and 2 were isolated from Asterina pectinifera for the first time,while compound 4 was first obtained from Asterina pectinifera of China Sea.
Objective : To study the chemical constituents of marine starfish Asterina pectinifera. Methods : The constituents were separated and purified by column chromatography with silica gel and sephadex LH-20.Their structures were identified by spectrum and physical data. Results :Seven compounds were identified as : 3-(octadecyloxy)-1,2-Propanediol(1),5α-Cholest-7-en-3β-ol(2),asterosapoin P1(3),5α-cholestane-3β,6α,7α,8,15α,16β,26-heptol(4),5α-cholestane-3β,4β,6α,7α,8,15β,16β,26-octol(5),tryptophan(6),phenylalanine(7). Conclusions :Compounds 1 and 2 were isolated from Asterina pectinifera for the first time,while compound 4 was first obtained from Asterina pectinifera of China Sea.
2009, 27(2): 101-103.
Abstract:
Objective : To investigate the chemical constituents of Rosa cymosa. Methods : Multi-chromatographic methods were employed for the isolation and purification of Rosa cymosa.The structures were identified on the basis of chemical evidence and spectral data. Results :Six compounds were isolated and identified as β-sitosterol (1),2α-hydroxyursolic acid(2),euscaphic acid(3),daucosterol(4),kaemferol-3-O-β-D-glucoside(5),potengriffioside A(6). Conclusion :Compounds 1～6 were all isolated from Rosa cymosa for the first time.
Objective : To investigate the chemical constituents of Rosa cymosa. Methods : Multi-chromatographic methods were employed for the isolation and purification of Rosa cymosa.The structures were identified on the basis of chemical evidence and spectral data. Results :Six compounds were isolated and identified as β-sitosterol (1),2α-hydroxyursolic acid(2),euscaphic acid(3),daucosterol(4),kaemferol-3-O-β-D-glucoside(5),potengriffioside A(6). Conclusion :Compounds 1～6 were all isolated from Rosa cymosa for the first time.
2009, 27(2): 104-106,110.
Abstract:
Objective : To study the method of tracing the floating tablet with isotope in the stomach and discuss the security and the influence factors to the floating tablet detained in the stomach. Methods :The 99mTc as the sign was added to the raw material of tablet by ECT.The images in the stomach were checked and the residual quantity of isotope in intestine,urine and excrement was traced. Results :The image of tablet in the stomach was clear.The radioactivity can not traced in excreta of volunteer 48 h later.After meal,the detained time of floating in stomach can be prolonged. Conclusion :The floating tablets could be traced with 99mTc and collected the images with ECT.It is safe if only control the total of isotope.The major factor of affecting the detained time of the floating tablets is the food in the stomach.
Objective : To study the method of tracing the floating tablet with isotope in the stomach and discuss the security and the influence factors to the floating tablet detained in the stomach. Methods :The 99mTc as the sign was added to the raw material of tablet by ECT.The images in the stomach were checked and the residual quantity of isotope in intestine,urine and excrement was traced. Results :The image of tablet in the stomach was clear.The radioactivity can not traced in excreta of volunteer 48 h later.After meal,the detained time of floating in stomach can be prolonged. Conclusion :The floating tablets could be traced with 99mTc and collected the images with ECT.It is safe if only control the total of isotope.The major factor of affecting the detained time of the floating tablets is the food in the stomach.
2009, 27(2): 107-110.
Abstract:
Objective : Design and synthesis of novel triazole antifungal derivatives based on the structure of fluconazole. Methods : A 4-carboxylic ester substituted benzyl group introduced and a series title compounds synthesized.All of them were confirmed by MS,1H-NMR,etal.The antifungal activities were also evaluated against the eight tested pathogenic fungi. Results :Fifteen title compounds were synthesized.All title compounds exhibited activity against fungi tested to some extent.Compounds(1),(2)and(3)exhibited strong antifungal activities against eight fungi comparable to the control drug itraconazole except Aspergillus fumigatus. Conclusions :A benzyl moiety with a group of shorter length substituted on 4-position is required for high activity.
Objective : Design and synthesis of novel triazole antifungal derivatives based on the structure of fluconazole. Methods : A 4-carboxylic ester substituted benzyl group introduced and a series title compounds synthesized.All of them were confirmed by MS,1H-NMR,etal.The antifungal activities were also evaluated against the eight tested pathogenic fungi. Results :Fifteen title compounds were synthesized.All title compounds exhibited activity against fungi tested to some extent.Compounds(1),(2)and(3)exhibited strong antifungal activities against eight fungi comparable to the control drug itraconazole except Aspergillus fumigatus. Conclusions :A benzyl moiety with a group of shorter length substituted on 4-position is required for high activity.
2009, 27(2): 111-114.
Abstract:
Objective : To investigate the anti-inflammatory and analgesic effects of extracts of Solanum lyratum Thumb. Methods : The anti-inflammatory and analgesic effects of alcohol extract and water extract from Solanum lyratum Thumb were observed by means of acetic acid inducing mouse writhing model,hot plate method,dimethylbenzene inducing ear edema model and carrageenan inducing rat voix pedis swell model. Results :It was found that alcohol extract and water extract from Solanum lyratum Thumb significantly reducing the writhing frequency induced by acetic acid,markedly increasing the pain threshold in mice induced by hot plate,evidently suppressing the mouse's ear edema induced by dimethylbenzene and significantly mitigating the rat voix pedis swell inducing by carrageenan.The role of water extract was better than which of alcohol extract. Conclusions :The water extract from solanum lyratum thumb has notable effects on anti-inflammation and analgesia.
Objective : To investigate the anti-inflammatory and analgesic effects of extracts of Solanum lyratum Thumb. Methods : The anti-inflammatory and analgesic effects of alcohol extract and water extract from Solanum lyratum Thumb were observed by means of acetic acid inducing mouse writhing model,hot plate method,dimethylbenzene inducing ear edema model and carrageenan inducing rat voix pedis swell model. Results :It was found that alcohol extract and water extract from Solanum lyratum Thumb significantly reducing the writhing frequency induced by acetic acid,markedly increasing the pain threshold in mice induced by hot plate,evidently suppressing the mouse's ear edema induced by dimethylbenzene and significantly mitigating the rat voix pedis swell inducing by carrageenan.The role of water extract was better than which of alcohol extract. Conclusions :The water extract from solanum lyratum thumb has notable effects on anti-inflammation and analgesia.
2009, 27(2): 115-117,121.
Abstract:
Objective : To develop a RP-HPLC method for determination of the rabeprazole sodium in human plasma. Methods : The chromatographic separation was carried out on Dikma Diamonsil C18 column(4.6 mm×250 mm,5μm),the mobile phase was 0.1 mol/L ammonium acetate buffer(pH adjusted to 7.81 with sodium hydroxide solution)-acetonitrile(7228) and the flow rate was 1.5 mL/min with UV detection(284 nm).After extraction once from plasma with ethyl acetate,RPZ was evaporated to dryness at 40 ℃ under a nitrogen stream. Results :The linear range was 5～1 600 ng/mL,r=0.999 1.The extraction recovery of raberazole was78.45 %～89.56 %,recovery of the method was within 95.16 %～97.52 %,and within-day and between-day RSD 6.25% and 8.23%,respectrely. Conclusion :The method is sensitive,specific and reproducible for the determination of rabeprazole sodium concentration in human plasma.It is very suitable for pharmacokinetics study of rabeprazole.
Objective : To develop a RP-HPLC method for determination of the rabeprazole sodium in human plasma. Methods : The chromatographic separation was carried out on Dikma Diamonsil C18 column(4.6 mm×250 mm,5μm),the mobile phase was 0.1 mol/L ammonium acetate buffer(pH adjusted to 7.81 with sodium hydroxide solution)-acetonitrile(7228) and the flow rate was 1.5 mL/min with UV detection(284 nm).After extraction once from plasma with ethyl acetate,RPZ was evaporated to dryness at 40 ℃ under a nitrogen stream. Results :The linear range was 5～1 600 ng/mL,r=0.999 1.The extraction recovery of raberazole was78.45 %～89.56 %,recovery of the method was within 95.16 %～97.52 %,and within-day and between-day RSD 6.25% and 8.23%,respectrely. Conclusion :The method is sensitive,specific and reproducible for the determination of rabeprazole sodium concentration in human plasma.It is very suitable for pharmacokinetics study of rabeprazole.
2009, 27(2): 118-121.
Abstract:
Objective : To search for the optimum formula ratio for the self-emulsifying drug delivery systems of adefovir dipivoxil. Methods : The recipe of the self-emulsifying drug delivery systems of adefovir dipivoxil formation was optimized by studying the pseudo-ternary phase diagram,the self-emulsifying rate,particle size.Adefovir was successfully embedded in the SEDDS emulsion. Results :The optimized prescription was tricaprylin as oil,Cremophor EL35 as surfactant,with the oil and the surfactant ratio was 73. Conclusions :The preliminary adefovir dipivoxil Self-emulsifying prepartion has been prepared.
Objective : To search for the optimum formula ratio for the self-emulsifying drug delivery systems of adefovir dipivoxil. Methods : The recipe of the self-emulsifying drug delivery systems of adefovir dipivoxil formation was optimized by studying the pseudo-ternary phase diagram,the self-emulsifying rate,particle size.Adefovir was successfully embedded in the SEDDS emulsion. Results :The optimized prescription was tricaprylin as oil,Cremophor EL35 as surfactant,with the oil and the surfactant ratio was 73. Conclusions :The preliminary adefovir dipivoxil Self-emulsifying prepartion has been prepared.
2009, 27(2): 123-125.
Abstract:
Objective : To observe the protective effect of Compound Yinhu decoction on acute liver injury induced by carbon tetrachloride(CCl4) in mice. Methods : Acute liver injury was established by intraperitoneal injection of CCl4.Compound Yinhu decotion was tested as subjects drug and bifendate as a positive control drug.The liver index(LI),the levels of serum alanine amino-transferase(ALT) and aspartate amino-transferase(AST),the superoxide dismutase(SOD) and molondialdehyde(MDA) levels in the liver tissue were measured and the protective effect of Compound Yinhu decotion on liver injury was observed. Results :Compound Yinhu decotion could significantly reduce serum ALT and AST levels,increase liver SOD level and decrease liver MDA level.It could also reduce the liver cell morphological injury. Conclusions :Compound Yinhu decotion has some protective effect on acute liver injury induced by CCl4 in mice.
Objective : To observe the protective effect of Compound Yinhu decoction on acute liver injury induced by carbon tetrachloride(CCl4) in mice. Methods : Acute liver injury was established by intraperitoneal injection of CCl4.Compound Yinhu decotion was tested as subjects drug and bifendate as a positive control drug.The liver index(LI),the levels of serum alanine amino-transferase(ALT) and aspartate amino-transferase(AST),the superoxide dismutase(SOD) and molondialdehyde(MDA) levels in the liver tissue were measured and the protective effect of Compound Yinhu decotion on liver injury was observed. Results :Compound Yinhu decotion could significantly reduce serum ALT and AST levels,increase liver SOD level and decrease liver MDA level.It could also reduce the liver cell morphological injury. Conclusions :Compound Yinhu decotion has some protective effect on acute liver injury induced by CCl4 in mice.
2009, 27(2): 126-128.
Abstract:
Objective : To investigate the anti-depressant effects of Akebia fruit alcohol extract and to explore its mechanism. Methods :Tail suspension model and force-swim model of mice were used to observe the effect of Akebia fruit alcohol extract on relieving depression.The mechanism was examined by inhibiting reuptake of monoamine neurotransmitters. Results :Akebia fruit alcohol extract can reduced the immobility time in both of the tail suspension test and the forced swim test of mice.Akebia fruit alcohol extract significantly increased the rate of death of injecting dopamine and enhanced the apomorphine-induced stereotypy,could increase the head-twitch response evoked by 5-HTP,but it had no direct activation on reuptake of noradrenalin. Conclusion :Akebia fruit alcohol extract has antidepressant effects,and its mechanism may be related to the reinforcement of 5-HT nerves system and DA nerves system.
Objective : To investigate the anti-depressant effects of Akebia fruit alcohol extract and to explore its mechanism. Methods :Tail suspension model and force-swim model of mice were used to observe the effect of Akebia fruit alcohol extract on relieving depression.The mechanism was examined by inhibiting reuptake of monoamine neurotransmitters. Results :Akebia fruit alcohol extract can reduced the immobility time in both of the tail suspension test and the forced swim test of mice.Akebia fruit alcohol extract significantly increased the rate of death of injecting dopamine and enhanced the apomorphine-induced stereotypy,could increase the head-twitch response evoked by 5-HTP,but it had no direct activation on reuptake of noradrenalin. Conclusion :Akebia fruit alcohol extract has antidepressant effects,and its mechanism may be related to the reinforcement of 5-HT nerves system and DA nerves system.
2009, 27(2): 129-131.
Abstract:
Objective :To study the apoptosis and mechanism of arsenicals on human gastric carcinoma cells. Methods : MTT,FCM and RT-PCR tests were used to investigate of apoptosis levels induced by arsenic trioxide and the effect on LRP、C-myc expression. Results :1.0 μmol/L and 2.0 μmol/L arsenic trioxide can decrease LRP、C-myc expression. Conclusions :Low concentrations may improve apoptosis of gastric cancer by decreasing LRP、C-myc expression.As2O3 may be used to treat gastric cancer but the results are no better than cisplatin.
Objective :To study the apoptosis and mechanism of arsenicals on human gastric carcinoma cells. Methods : MTT,FCM and RT-PCR tests were used to investigate of apoptosis levels induced by arsenic trioxide and the effect on LRP、C-myc expression. Results :1.0 μmol/L and 2.0 μmol/L arsenic trioxide can decrease LRP、C-myc expression. Conclusions :Low concentrations may improve apoptosis of gastric cancer by decreasing LRP、C-myc expression.As2O3 may be used to treat gastric cancer but the results are no better than cisplatin.
2009, 27(2): 132-133,145.
Abstract:
Objective : To study the stability of the mixture of potassium sodium dehydroandrographolide succinate for injection with cefuroxime sodium injection in 0.9% sodium chloride injection. Methods : HPLC method was adopted.The contents were determined after mixing of potassium sodium dehydroandrographolide succinate with Cefuroxime injection in 0.9% sodium chloride injection under the room temperature within 8 hours,and the appearance of the solution was observed and its pH value was determined. Results :No significant differences were found in terms of pH value,but the colour gradually deepens as time goes on.The relative percentage of cefuroxime sodium drops to below 95% after 5 h,and the degradation product peak area accounts for the total peak area percentage to surpass 1% after 4 h. Conclusions :The mixture of potassium sodium dehydroandrographolide succinate with cefuroxime sodium injection in 0.9% sodium chloride injection can be used in NS under room temperature,but must be injected during 4 hours.
Objective : To study the stability of the mixture of potassium sodium dehydroandrographolide succinate for injection with cefuroxime sodium injection in 0.9% sodium chloride injection. Methods : HPLC method was adopted.The contents were determined after mixing of potassium sodium dehydroandrographolide succinate with Cefuroxime injection in 0.9% sodium chloride injection under the room temperature within 8 hours,and the appearance of the solution was observed and its pH value was determined. Results :No significant differences were found in terms of pH value,but the colour gradually deepens as time goes on.The relative percentage of cefuroxime sodium drops to below 95% after 5 h,and the degradation product peak area accounts for the total peak area percentage to surpass 1% after 4 h. Conclusions :The mixture of potassium sodium dehydroandrographolide succinate with cefuroxime sodium injection in 0.9% sodium chloride injection can be used in NS under room temperature,but must be injected during 4 hours.
2009, 27(2): 134-136.
Abstract:
Objective : To develop a HPLC method for determinating content of triptolide in different parts of Tripterygium Hypoglaucum(Levl.)Hutch from Guangxi in China. Methods : The determination of triptolide was performed by reverse-HPLC on C18 column(250 mm×4.6 mm) using methanol-water(45∶55) as mobile phase with a flow rate of 1 mL/min.The detecting wavelength was at 217 nm and the injection volume was 20 μL. Results : The linear range of triptolide was 0.5～10 μg/mL(r=0.999 9).The average recovery was 99.61%,and RSD was 1.68%.The average content of triptolide in Tripterygium Hypoglaucum(Levl.)Hutch was 14.98 μg/g in root and 3.64 μg/g in stem. Conclusions :The established method is simple,convenient,accurate,reproducible,and can be applied to the quality control of triptolide in Tripterygium hypoglaucum(Levl.)Hutch.
Objective : To develop a HPLC method for determinating content of triptolide in different parts of Tripterygium Hypoglaucum(Levl.)Hutch from Guangxi in China. Methods : The determination of triptolide was performed by reverse-HPLC on C18 column(250 mm×4.6 mm) using methanol-water(45∶55) as mobile phase with a flow rate of 1 mL/min.The detecting wavelength was at 217 nm and the injection volume was 20 μL. Results : The linear range of triptolide was 0.5～10 μg/mL(r=0.999 9).The average recovery was 99.61%,and RSD was 1.68%.The average content of triptolide in Tripterygium Hypoglaucum(Levl.)Hutch was 14.98 μg/g in root and 3.64 μg/g in stem. Conclusions :The established method is simple,convenient,accurate,reproducible,and can be applied to the quality control of triptolide in Tripterygium hypoglaucum(Levl.)Hutch.
2009, 27(2): 154-156.
Abstract:
Objective :To develop a war medicine's modular management and search system for PDA,which can be used by military hospitals. Methods :The system is based on Microsoft .NETCompact Framework 2.0 and built by using the newest technology such as C#.NET, SQL Server2005 sp2 and SQL Mobile 2005. Results :With this system,the users can easily look up drugs'stability and compatibility and manage war medicine better. Conclusion :I The system can improve the efficiency of war medicine's management.
Objective :To develop a war medicine's modular management and search system for PDA,which can be used by military hospitals. Methods :The system is based on Microsoft .NETCompact Framework 2.0 and built by using the newest technology such as C#.NET, SQL Server2005 sp2 and SQL Mobile 2005. Results :With this system,the users can easily look up drugs'stability and compatibility and manage war medicine better. Conclusion :I The system can improve the efficiency of war medicine's management.
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