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Volume 27 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(2): 115-117,121

CHEN Hong, DING Xue-ying, GAO Jing, GAO Shen. Determination of rabeprazole sodium in human plasma by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 115-117,121.
Citation: CHEN Hong, DING Xue-ying, GAO Jing, GAO Shen. Determination of rabeprazole sodium in human plasma by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 115-117,121.
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Determination of rabeprazole sodium in human plasma by RP-HPLC

  • Department of Pharmaceutics, Pharmacy College, Secondary Military Medical University, Shanghai 200433, China

  • Received Date: 2009-01-08
  • Objective : To develop a RP-HPLC method for determination of the rabeprazole sodium in human plasma. Methods : The chromatographic separation was carried out on Dikma Diamonsil C18 column(4.6 mm×250 mm,5μm),the mobile phase was 0.1 mol/L ammonium acetate buffer(pH adjusted to 7.81 with sodium hydroxide solution)-acetonitrile(7228) and the flow rate was 1.5 mL/min with UV detection(284 nm).After extraction once from plasma with ethyl acetate,RPZ was evaporated to dryness at 40 ℃ under a nitrogen stream. Results :The linear range was 5～1 600 ng/mL,r=0.999 1.The extraction recovery of raberazole was78.45 %～89.56 %,recovery of the method was within 95.16 %～97.52 %,and within-day and between-day RSD 6.25% and 8.23%,respectrely. Conclusion :The method is sensitive,specific and reproducible for the determination of rabeprazole sodium concentration in human plasma.It is very suitable for pharmacokinetics study of rabeprazole.
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Determination of rabeprazole sodium in human plasma by RP-HPLC

  • Department of Pharmaceutics, Pharmacy College, Secondary Military Medical University, Shanghai 200433, China
  • Received Date: 2009-01-08

Abstract: Objective : To develop a RP-HPLC method for determination of the rabeprazole sodium in human plasma. Methods : The chromatographic separation was carried out on Dikma Diamonsil C18 column(4.6 mm×250 mm,5μm),the mobile phase was 0.1 mol/L ammonium acetate buffer(pH adjusted to 7.81 with sodium hydroxide solution)-acetonitrile(7228) and the flow rate was 1.5 mL/min with UV detection(284 nm).After extraction once from plasma with ethyl acetate,RPZ was evaporated to dryness at 40 ℃ under a nitrogen stream. Results :The linear range was 5～1 600 ng/mL,r=0.999 1.The extraction recovery of raberazole was78.45 %～89.56 %,recovery of the method was within 95.16 %～97.52 %,and within-day and between-day RSD 6.25% and 8.23%,respectrely. Conclusion :The method is sensitive,specific and reproducible for the determination of rabeprazole sodium concentration in human plasma.It is very suitable for pharmacokinetics study of rabeprazole.

CHEN Hong, DING Xue-ying, GAO Jing, GAO Shen. Determination of rabeprazole sodium in human plasma by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 115-117,121.
Citation: CHEN Hong, DING Xue-ying, GAO Jing, GAO Shen. Determination of rabeprazole sodium in human plasma by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 115-117,121.
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