Determination of triptolide in Tripterygium hypoglaucum by HPLC
- Received Date: 2008-05-04
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Key words:
- Tripterygium hypoglaucum(Levl.)Hutch /
- triptolide /
- HPLC
Abstract: Objective : To develop a HPLC method for determinating content of triptolide in different parts of Tripterygium Hypoglaucum(Levl.)Hutch from Guangxi in China. Methods : The determination of triptolide was performed by reverse-HPLC on C18 column(250 mm×4.6 mm) using methanol-water(45∶55) as mobile phase with a flow rate of 1 mL/min.The detecting wavelength was at 217 nm and the injection volume was 20 μL. Results : The linear range of triptolide was 0.5~10 μg/mL(r=0.999 9).The average recovery was 99.61%,and RSD was 1.68%.The average content of triptolide in Tripterygium Hypoglaucum(Levl.)Hutch was 14.98 μg/g in root and 3.64 μg/g in stem. Conclusions :The established method is simple,convenient,accurate,reproducible,and can be applied to the quality control of triptolide in Tripterygium hypoglaucum(Levl.)Hutch.
Citation: | CHEN Yue-xiang, QIAO Yan, QIAO Hua-min, YAO Jian-zhong. Determination of triptolide in Tripterygium hypoglaucum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 134-136. |