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Volume 27 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(2): 134-136

CHEN Yue-xiang, QIAO Yan, QIAO Hua-min, YAO Jian-zhong. Determination of triptolide in Tripterygium hypoglaucum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 134-136.
Citation: CHEN Yue-xiang, QIAO Yan, QIAO Hua-min, YAO Jian-zhong. Determination of triptolide in Tripterygium hypoglaucum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 134-136.
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Determination of triptolide in Tripterygium hypoglaucum by HPLC

  • 1.

    Department of pharmacy, The 181th Hospital of PLA, Guilin 541002, China; School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 2.

    Department of pharmacy, The 181th Hospital of PLA, Guilin 541002, Chin

  • 3.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2008-05-04
  • Objective : To develop a HPLC method for determinating content of triptolide in different parts of Tripterygium Hypoglaucum(Levl.)Hutch from Guangxi in China. Methods : The determination of triptolide was performed by reverse-HPLC on C18 column(250 mm×4.6 mm) using methanol-water(45∶55) as mobile phase with a flow rate of 1 mL/min.The detecting wavelength was at 217 nm and the injection volume was 20 μL. Results : The linear range of triptolide was 0.5～10 μg/mL(r=0.999 9).The average recovery was 99.61%,and RSD was 1.68%.The average content of triptolide in Tripterygium Hypoglaucum(Levl.)Hutch was 14.98 μg/g in root and 3.64 μg/g in stem. Conclusions :The established method is simple,convenient,accurate,reproducible,and can be applied to the quality control of triptolide in Tripterygium hypoglaucum(Levl.)Hutch.
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  • 1. 

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Determination of triptolide in Tripterygium hypoglaucum by HPLC

  • 1. Department of pharmacy, The 181th Hospital of PLA, Guilin 541002, China; School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • 2. Department of pharmacy, The 181th Hospital of PLA, Guilin 541002, Chin
  • 3. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2008-05-04

Abstract: Objective : To develop a HPLC method for determinating content of triptolide in different parts of Tripterygium Hypoglaucum(Levl.)Hutch from Guangxi in China. Methods : The determination of triptolide was performed by reverse-HPLC on C18 column(250 mm×4.6 mm) using methanol-water(45∶55) as mobile phase with a flow rate of 1 mL/min.The detecting wavelength was at 217 nm and the injection volume was 20 μL. Results : The linear range of triptolide was 0.5～10 μg/mL(r=0.999 9).The average recovery was 99.61%,and RSD was 1.68%.The average content of triptolide in Tripterygium Hypoglaucum(Levl.)Hutch was 14.98 μg/g in root and 3.64 μg/g in stem. Conclusions :The established method is simple,convenient,accurate,reproducible,and can be applied to the quality control of triptolide in Tripterygium hypoglaucum(Levl.)Hutch.

CHEN Yue-xiang, QIAO Yan, QIAO Hua-min, YAO Jian-zhong. Determination of triptolide in Tripterygium hypoglaucum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 134-136.
Citation: CHEN Yue-xiang, QIAO Yan, QIAO Hua-min, YAO Jian-zhong. Determination of triptolide in Tripterygium hypoglaucum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(2): 134-136.
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