Expression and distribution of secretory phospholipase A2 in human skin melanoma cells and tissues
- Received Date: 2009-10-25
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Key words:
- secretory phospholipase A2 /
- melanoma /
- expression /
- distribution
Abstract: Objective :To investigate the expression and distribution of secretory phospholipase A2(sPLA2) in A375 human malignant melanoma cells and melanoma tissues compared with normal human skin cells and tissues. Methods :The expression of sPLA2 in the clear supernatant and intra-cellular of cells was detected by ELISA method and Western Blot method,respectively.Immunohistochemical method was used to investigate the expression and distribution of sPLA2 in the human skin tissues. Results :The levels of sPLA2 in the clear supernatant of A375 malignant melanoma cells was(177.27±13.57) pg/mL,which was significantly higher(P<0.05) than the group of human epidermal HaCaT cells(21.42±5.05) pg/ml and the group of dermal fibroblast cells(2.75±1.23) pg/mL,respectively.The ratio of sPLA2/GAPDH in the A375 cells was(7.03±1.31),which was also significantly higher(P<0.05) than the group of human epidermal HaCaT cells(1.45±0.37) and the group of dermal fibroblast cells(0.31±0.11),respectively.Many sPLA2 positive cells and granules were presented in the tumor tissues.In contrast,positive cells and granules above were unobserved in the normal human epidermal and dermal tissues. Conclusion :sPLA2 is expressed and distributed at higher levels in A375 human malignant melanoma cells and melanoma tissues compared with the normal skin cells and tissues,indicating the sPLA2 maybe a novel trigger for targeted drug delivery to skin melanoma.
Citation: | ZHANG Li-chao, ZHANG Yong-jia, GU Wei-ying, WANG Hui-jing, SHI Kou-rong. Expression and distribution of secretory phospholipase A2 in human skin melanoma cells and tissues[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 417-420. |