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CHEN Dan-xia, ZHOU Ting-ting, WANG Jia-jing, ZHANG Qiao-yan, FAN Guo-rong. Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 430-433.
Citation: CHEN Dan-xia, ZHOU Ting-ting, WANG Jia-jing, ZHANG Qiao-yan, FAN Guo-rong. Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 430-433.

Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC

  • Received Date: 2009-02-26
  • Objective :To establish a RP-HPLC method for separation and determination of active constituents in Fructus Cnidii from 10 different regions,and evaluate the quality of the Fructus Cnidii. Methods :DiamonsilTM C18(150mm×4.6 mm,5μm) was used with a mobile phase of ammonium acetate buffer(pH 4.0)/acetonitrile(50/50,,v/v) and a flow rate of 1.0 mL/min,at the detection wavelength of 320 nm. Results :The calibration curves were linear in the range of 4~100μg/mL for bergapten(r=0.9998),4~100 μg/mL for imperatorin(r=0.999 6),10~200 μg/mL for osthole(r=0.999 7),respectively.The average recoveries were 98.1%,92.5%,98.4%,with the RSD of 4.58%,5.73%,4.51%,respectively. Conclusion :The method is rapid,simple and accurate,and it can be suitable for the separation and determination of bergapten,imperatorin and osthole in Fructus Cnidii,quality control and geoherbalism research of Fructus Cnidii.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC

Abstract: Objective :To establish a RP-HPLC method for separation and determination of active constituents in Fructus Cnidii from 10 different regions,and evaluate the quality of the Fructus Cnidii. Methods :DiamonsilTM C18(150mm×4.6 mm,5μm) was used with a mobile phase of ammonium acetate buffer(pH 4.0)/acetonitrile(50/50,,v/v) and a flow rate of 1.0 mL/min,at the detection wavelength of 320 nm. Results :The calibration curves were linear in the range of 4~100μg/mL for bergapten(r=0.9998),4~100 μg/mL for imperatorin(r=0.999 6),10~200 μg/mL for osthole(r=0.999 7),respectively.The average recoveries were 98.1%,92.5%,98.4%,with the RSD of 4.58%,5.73%,4.51%,respectively. Conclusion :The method is rapid,simple and accurate,and it can be suitable for the separation and determination of bergapten,imperatorin and osthole in Fructus Cnidii,quality control and geoherbalism research of Fructus Cnidii.

CHEN Dan-xia, ZHOU Ting-ting, WANG Jia-jing, ZHANG Qiao-yan, FAN Guo-rong. Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 430-433.
Citation: CHEN Dan-xia, ZHOU Ting-ting, WANG Jia-jing, ZHANG Qiao-yan, FAN Guo-rong. Analysis of characteristic constituents in Fructus Cnidii extracts by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 430-433.

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