Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 28 Issue 3
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2010  >  28(3): 186-188

DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
Citation: DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
shu

Determination of trehalose in Candida albicans by HPLC-MS

  • 1.

    School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • 2.

    School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • Received Date: 2010-01-25
  • Rev Recd Date: 2010-03-08
  • Objective To establish a high performance liquid chromatography-mass spectrum(HPLC-MS) method to determine trehalose in the biofilm of Candida albicans. Methods Shisheido CAPCELL NH2(150 mm×4.6 mm,5 μm) was used in this method and the mobile phase is 75% acetonitrile and 25% pure water.MS detector with an ESI ion source in negative mode was used. Results It has a good separation of trehalose and internal standard,and there is no interference of endogenous impurity.Linear was from 0.05～50 μg/ml(r=0.999 3),recovery was in the range of 81.50% to 96.29% and the RSD of precision is less than 2.1 %. Conclusion This method is a simple method for quantification of trehalose in fungus.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2279) PDF downloads(585) Cited by()

Related
Proportional views

Determination of trehalose in Candida albicans by HPLC-MS

  • 1. School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • 2. School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • Received Date: 2010-01-25
  • Rev Recd Date: 2010-03-08

Abstract: Objective To establish a high performance liquid chromatography-mass spectrum(HPLC-MS) method to determine trehalose in the biofilm of Candida albicans. Methods Shisheido CAPCELL NH2(150 mm×4.6 mm,5 μm) was used in this method and the mobile phase is 75% acetonitrile and 25% pure water.MS detector with an ESI ion source in negative mode was used. Results It has a good separation of trehalose and internal standard,and there is no interference of endogenous impurity.Linear was from 0.05～50 μg/ml(r=0.999 3),recovery was in the range of 81.50% to 96.29% and the RSD of precision is less than 2.1 %. Conclusion This method is a simple method for quantification of trehalose in fungus.

DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
Citation: DONG Ling-ling, CAO Ying-ying, CHAI Yi-feng. Determination of trehalose in Candida albicans by HPLC-MS[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(3): 186-188.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return