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Volume 30 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2012  >  30(4): 305-306,315

HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.
Citation: SUN Wei-zhang, YU Bo-tao, JIANG Yan, ZHOU Mao-zai. Determination of glutathione and oxidized glutathione by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(4): 305-306,315.
shu

Determination of glutathione and oxidized glutathione by HPLC

  • 1.

    General Hospital of Chengdu Military Command, PET-CT center, Chengdu 610083, China

  • 2.

    General Hospital of Chengdu Military Command, Department of Pharmacy, Chengdu 610083, China

  • Received Date: 2012-03-12
  • Rev Recd Date: 2012-05-21
  • Objective To establish a method to determine GSH and GSSG by HPLC. Method The analytical column was Hypersil C18. The mobile phase consisted of 25m mol/L KH2PO4 (pH2.5):tetrahydrofuran (100:1). The flow rate was 1.0 ml/min. Column temperature was 30 ℃ and the detective wavelength was 230 nm. Result The calibration curve of GSH was linear within the range of 3.12 μg/ml～2490.0 μg/ml (r=0.9999) and the average recovery was 101.84%. The calibration curve of GSSG was linear within the range of 3.25 μg/ml～975.0 μg/ml (r=0.9999) and the average recovery was 97,84%. Conclusion The method was proved to be reliable and accurate,which could be used to determinate GSH and GSSG simultaneously.
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Determination of glutathione and oxidized glutathione by HPLC

  • 1. General Hospital of Chengdu Military Command, PET-CT center, Chengdu 610083, China
  • 2. General Hospital of Chengdu Military Command, Department of Pharmacy, Chengdu 610083, China
  • Received Date: 2012-03-12
  • Rev Recd Date: 2012-05-21

Abstract: Objective To establish a method to determine GSH and GSSG by HPLC. Method The analytical column was Hypersil C18. The mobile phase consisted of 25m mol/L KH2PO4 (pH2.5):tetrahydrofuran (100:1). The flow rate was 1.0 ml/min. Column temperature was 30 ℃ and the detective wavelength was 230 nm. Result The calibration curve of GSH was linear within the range of 3.12 μg/ml～2490.0 μg/ml (r=0.9999) and the average recovery was 101.84%. The calibration curve of GSSG was linear within the range of 3.25 μg/ml～975.0 μg/ml (r=0.9999) and the average recovery was 97,84%. Conclusion The method was proved to be reliable and accurate,which could be used to determinate GSH and GSSG simultaneously.

HE Hou-luo, HUANG Zhang-bei, SHENG Jia-yu, YUE Xiao-qiang, LI Min, XIN Hai-liang. Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 291-293.
Citation: SUN Wei-zhang, YU Bo-tao, JIANG Yan, ZHOU Mao-zai. Determination of glutathione and oxidized glutathione by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(4): 305-306,315.
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