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Volume 30 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2012  >  30(4): 307-309

WU Wen-fei, ZHANG Li-bin, LI Yu-pin. Determination of buddleoside in Zhikuoyangyin granules by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(4): 307-309.
Citation: WU Wen-fei, ZHANG Li-bin, LI Yu-pin. Determination of buddleoside in Zhikuoyangyin granules by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(4): 307-309.
shu

Determination of buddleoside in Zhikuoyangyin granules by HPLC

  • Department of Pharmacy, Shanghai Pulmonary Hospital Affiliated to Tongji University, Shanghai 200433, China

  • Received Date: 2011-12-03
  • Rev Recd Date: 2011-12-31
  • Objective To establish a high performance liquid chromatography method for the determination of buddleoside in Zhikuoyangyin granules. Methods The HPLC method was performed on a DIKMA Platisil ODS column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of methanol-water-acetic acid (55:44:2). The flow rate was 1.0 ml/min. The sample injection volume was 20 μl. The detective wavelength was 326 nm. Results The calibration curve of buddleoside showed good linear response ranged from 2.95 to 98.3μg/ml with r=0.9999 (n=5). The average recovery of spiked samples for buddleoside was 100.2% while the relative standard deviation for repetitions was 0.96%(n=15). Conclusion The method was sirepie, sensitive,reliable and repeatable,and could be used for the quantitative determination of buddleoside in Zhikuoyangyin granules.
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Determination of buddleoside in Zhikuoyangyin granules by HPLC

  • Department of Pharmacy, Shanghai Pulmonary Hospital Affiliated to Tongji University, Shanghai 200433, China
  • Received Date: 2011-12-03
  • Rev Recd Date: 2011-12-31

Abstract: Objective To establish a high performance liquid chromatography method for the determination of buddleoside in Zhikuoyangyin granules. Methods The HPLC method was performed on a DIKMA Platisil ODS column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of methanol-water-acetic acid (55:44:2). The flow rate was 1.0 ml/min. The sample injection volume was 20 μl. The detective wavelength was 326 nm. Results The calibration curve of buddleoside showed good linear response ranged from 2.95 to 98.3μg/ml with r=0.9999 (n=5). The average recovery of spiked samples for buddleoside was 100.2% while the relative standard deviation for repetitions was 0.96%(n=15). Conclusion The method was sirepie, sensitive,reliable and repeatable,and could be used for the quantitative determination of buddleoside in Zhikuoyangyin granules.

WU Wen-fei, ZHANG Li-bin, LI Yu-pin. Determination of buddleoside in Zhikuoyangyin granules by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(4): 307-309.
Citation: WU Wen-fei, ZHANG Li-bin, LI Yu-pin. Determination of buddleoside in Zhikuoyangyin granules by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(4): 307-309.
shu

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