HPLC法测定盐酸美沙酮含量
Determination of methadone hydrochloride by HPLC
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摘要: 本文采用高效液相色谱法,以盐酸布比卡因为内标,检测波长220nm,在Symmetry-C18柱上,以己腈-磷酸二氢钾(pH2.5)(3862,V/V)为流动相,测定盐酸美沙酮及其口服液的含量。方法平均回收率为100.17%,日内及日间的RSD均<1%。方法简便、快速、准确。Abstract: To estab1ish a HPLC method for determining methadone hydrochloride and methadone hydrochloride oral solution.Using a Symmetry-C18 Chlumn,a mixture of acetoni trile-monobasic potassium phosphate(pH2.5)(38:62,V/V)as mobile phase and detected at 220nm bupivacaine hydrochloride was used as internal standard.The average recovery was 100.17%.The RSD for the inter inter and intra-day were all less than 1%.The method is simple,rapid and accurate.
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Key words:
- methadone hydrochloride /
- methadone hydrochloride oral solutinn /
- HPLC
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