紫云英蜜中总糖及酸度的测定
Determination of total sugar and acidity in milk vetch honey
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摘要: 目的: 探讨紫云英蜜总糖含量和酸度的测定方法。 方法: 利用紫外分光光度法测定总糖的含量;电位滴定法测定酸度。 结果: 紫云英蜜总糖含量为541 mg/g,在26.2~131μg范围内呈良好的线性关系,r=0.998,平均回收率为102.1%,RSD=2.79%。测定紫云英蜜酸度时,避免了因指示剂使用而带来的误差,提高了测定准确度,RSD为0.98%~2.12%。 结论: 该方法简便准确,可靠,可作为紫云英蜜的质量分析方法。Abstract: Objective To establish the method for the quantitative determination of total sugar and acidity in milk vetch honey. Methods Ultraviolet spectrophotometry was used to determine the content of total sugar in honey.Potentionmetric titration was used to analyze the total acidity in the honey. Results The total sugar in honey was 541 mg/g which showed a good linear relationship in the range of 26.2～131 μg(r=0.998).The average recovery was 102.1% and RSD was 2.79%.Potentiometric titration was used to control the response end point to avoid indicator errors when the total acidity was determinatd.The accuracy was improve and RSD was 0.92%～2.27%. Conclusion The method is simple,convenient and reliable which could be used for the quality control.
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Key words:
- milk vetch honey /
- total sugar /
- UV-spectrophotometer /
- acidity /
- potentionmetric titration
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