运用GC-MS对三种不同方法提取的艾叶挥发油成分的比较分析
Analysis of volatile oil from Artemisia argyi Levi.et Vant extracted by three different methods by GC-MS
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摘要: 目的 运用GC-MS对不同提取方法所得的艾叶挥发油成分进行比较分析.方法 分别采用水蒸气蒸馏,正己烷回流,静态顶空提取艾叶挥发油,运用气相色谱-质谱联用技术,结合NIST05数据库对分离的化合物进行鉴定,并应用峰面积归一化法计算各成分的相对含量.结果 3种方法共鉴别出艾叶中80个挥发油成分,其中静态顶空进样59个,水蒸气蒸馏37个,正己烷回流31个.结论 结合水蒸气蒸馏和静态顶空进样两种方法可以建立更全面的艾叶挥发性成分GC-MS表征体系;静态顶空进样在挥发油成分鉴别上存在一定优势.Abstract: Objective To analyze the the compositions of volatile oil from Artemisia argyi Levi.et Vant extracted by three different methods by GC-MS.Methods The volatile oil from Artemisia argyi Levi.et Vant were extracted by Steam distillation,Hexane reflux extraction and Static headspace. GC-MS was used for the separation and identification. Results By the three methods, 80 compounds were identified.59 components were extracted by static headspace, 37 by steam distillation and 31 by hexane reflux extraction.Conclusion Combination of steam distillation and static headspace, sampling was feasible to establish GC-MS representation system for Artemisia argyi Levi.et Vant;there were certain advanlages of static headspace in determination of volatile oil contant.
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Key words:
- Artemisia argyi Levi.et Vant /
- GC-MS /
- static headspace /
- eucalyptol
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