温经汤高效液相色谱指纹图谱的建立

柴瑞平; 路娟; 赵颖; 王晓静; 吕欣锴; 陈曦

doi:10.3969/j.issn.1006-0111.2018.06.006

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温经汤高效液相色谱指纹图谱的建立

柴瑞平, 路娟, 赵颖, 王晓静, 吕欣锴, 陈曦

文章导航 > 药学实践与服务 > 2018 > 36(6): 503-506

柴瑞平, 路娟, 赵颖, 王晓静, 吕欣锴, 陈曦. 温经汤高效液相色谱指纹图谱的建立[J]. 药学实践与服务, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006

引用本文:

柴瑞平, 路娟, 赵颖, 王晓静, 吕欣锴, 陈曦. 温经汤高效液相色谱指纹图谱的建立[J]. 药学实践与服务, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006

CHAI Ruiping, LU Juan, ZHAO Ying, WANG Xiaojing, LÜ Xinkai, CHEN Xi. Establishment of HPLC fingerprint for Wenjing Tang[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006

Citation:

CHAI Ruiping, LU Juan, ZHAO Ying, WANG Xiaojing, LÜ Xinkai, CHEN Xi. Establishment of HPLC fingerprint for Wenjing Tang[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006

温经汤高效液相色谱指纹图谱的建立

doi: 10.3969/j.issn.1006-0111.2018.06.006

1.
中国医学科学院药用植物研究所, 北京 100193
2.
中国医学科学院药用植物研究所, 北京 100193;山东中医药大学, 山东济南 250355

基金项目: 国家自然科学基金面上项目（No.81673667）；中国医学科学院医学与健康科技创新工程经费资助（2016-I2M-3-015；2017-I2M-B&R-09）；云南省中青年学术带头人后备人才项目（2013HB099）

Establishment of HPLC fingerprint for Wenjing Tang

1.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China
2.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;Shandong University of Chinese Medicine, Jinan 250355, China

摘要: 目的建立古代经典名方温经汤HPLC-DAD指纹图谱检测方法。方法采用RP-HPLC-DAD法，利用Waters Xselect HSS T₃色谱柱（4.6 mm×250 mm，5 μm），以乙腈-0.1%甲酸水溶液作为流动相进行梯度洗脱，柱温25℃，流速0.8 ml/min，波长254 nm，选取甘草酸铵为参照峰，分析10批次温经汤水煎液的HPLC指纹图谱，并使用中药色谱指纹图谱相似度评价系统对指纹图谱相似度进行评价。结果在温经汤水煎液指纹图谱中标定了39个共有峰，指认了6个共有峰，10批样品指纹图谱相似度均>0.98，不同批次间差异较小。结论该方法灵敏度高、稳定性强，数据准确可靠，基本体现了温经汤的整体化学成分特征，可用于温经汤开发的质量控制。
- 温经汤 /
- 指纹图谱 /
- 高效液相色谱法 /
- 相似度
Abstract: Objective To establish chromatographic fingerprint for Wenjing Tang by HPLC-DAD. Methods The fingerprint was determined by Waters Xselect HSS T₃ column (4.6 mm×250 mm,5μm) with acetoniteile-0.1% formic acid solution as mobile phase for gradient elution. The temperature of column was 25℃ at a flow rate of 0.8 ml·min^-1. The detective wavelength was set at 254 nm and ammonium glycyrrhizinate was selected as the reference peak. The HPLC fingerprints of 10 batches of Wenjing Tang were analyzed and the similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine. Results There were 39 common peaks in the HPLC fingerprints of Wenjing Tang and 6 common peaks were determined by the relative time of the standard substance. The similarities of 10 batches were all above 0.98 and the deviations among these batches are very small. Conclusion The HPLC-DAD fingerprint method for Wenjing Tang is accurate, stable and reliable. The fingerprint is the characteristic of the overall chemical components in Wenjing Tang. It can be used as an effective method for the quality control of Wenjing Tang.
- Wenjing Tang /
- fingerprint /
- HPLC /
- similarity

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温经汤高效液相色谱指纹图谱的建立

doi: 10.3969/j.issn.1006-0111.2018.06.006

1. 中国医学科学院药用植物研究所, 北京 100193

2. 中国医学科学院药用植物研究所, 北京 100193;山东中医药大学, 山东济南 250355

收稿日期: 2017-12-12
修回日期: 2018-06-21

关键词:

温经汤 /

指纹图谱 /

高效液相色谱法 /

相似度

摘要: 目的建立古代经典名方温经汤HPLC-DAD指纹图谱检测方法。方法采用RP-HPLC-DAD法，利用Waters Xselect HSS T₃色谱柱（4.6 mm×250 mm，5 μm），以乙腈-0.1%甲酸水溶液作为流动相进行梯度洗脱，柱温25℃，流速0.8 ml/min，波长254 nm，选取甘草酸铵为参照峰，分析10批次温经汤水煎液的HPLC指纹图谱，并使用中药色谱指纹图谱相似度评价系统对指纹图谱相似度进行评价。结果在温经汤水煎液指纹图谱中标定了39个共有峰，指认了6个共有峰，10批样品指纹图谱相似度均>0.98，不同批次间差异较小。结论该方法灵敏度高、稳定性强，数据准确可靠，基本体现了温经汤的整体化学成分特征，可用于温经汤开发的质量控制。

English Abstract

Establishment of HPLC fingerprint for Wenjing Tang

1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China

2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;Shandong University of Chinese Medicine, Jinan 250355, China

Received Date: 2017-12-12
Rev Recd Date: 2018-06-21

Keywords:

Abstract: Objective To establish chromatographic fingerprint for Wenjing Tang by HPLC-DAD. Methods The fingerprint was determined by Waters Xselect HSS T₃ column (4.6 mm×250 mm,5μm) with acetoniteile-0.1% formic acid solution as mobile phase for gradient elution. The temperature of column was 25℃ at a flow rate of 0.8 ml·min^-1. The detective wavelength was set at 254 nm and ammonium glycyrrhizinate was selected as the reference peak. The HPLC fingerprints of 10 batches of Wenjing Tang were analyzed and the similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine. Results There were 39 common peaks in the HPLC fingerprints of Wenjing Tang and 6 common peaks were determined by the relative time of the standard substance. The similarities of 10 batches were all above 0.98 and the deviations among these batches are very small. Conclusion The HPLC-DAD fingerprint method for Wenjing Tang is accurate, stable and reliable. The fingerprint is the characteristic of the overall chemical components in Wenjing Tang. It can be used as an effective method for the quality control of Wenjing Tang.

柴瑞平, 路娟, 赵颖, 王晓静, 吕欣锴, 陈曦. 温经汤高效液相色谱指纹图谱的建立[J]. 药学实践与服务, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006

引用本文:

柴瑞平, 路娟, 赵颖, 王晓静, 吕欣锴, 陈曦. 温经汤高效液相色谱指纹图谱的建立[J]. 药学实践与服务, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006

Citation: