HPLC method to assay the content of psoralen and angelicin in Zhuanggu granules
doi: 10.3969/j.issn.1006-0111.2016.05.015
- Received Date: 2015-12-20
- Rev Recd Date: 2016-07-07
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Key words:
- HPLC /
- Zhuanggu granules /
- psoralen /
- angelicin
Abstract: Objective To develop a HPLC method for psoralenand angelicin content assay in Zhuanggu granules. Methods Agilent Zorbax C18 column(4.6 mm×250 mm, 5 μm) was used with methanol and water(45:55) as mobile phase. The detection wavelength was set at 245 nm. The flow rate was 1.0 ml/min and column temperature at 25 ℃. The injection volume was 10 μl.Psoralenand angelicin were extracted with 70% ethanol under water bath. Results The linearity was obtained over 3.75~40 μg/mlfor psoralenand angelicin. The RSDs were less than 2%. The average recovery was between 94% and 105%. Conclusion This simple and accurate HPLC method was suitable for the quality control of Zhuanggu granules.
Citation: | PU Jin, XU Liangyan, CHEN Ruohua. HPLC method to assay the content of psoralen and angelicin in Zhuanggu granules[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(5): 441-442. doi: 10.3969/j.issn.1006-0111.2016.05.015 |