Determination of matrine in Lefu oral liquid by HPLC

WU Xianglei; YUE Ming; HUANG Hao; LU Wenquan; PIAO Shujuan; ZHANG Feng

doi:10.3969/j.issn.1006-0111.2016.05.016

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Objective To establish a high performance liquid chromatography method for the determination of matrine in Lefu oral liquid. Methods The HPLC method was performed on a Diamonsil Platisil NH₂ column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-isopropyl alcohol-3% phosphoric acid solution (84:4:12). The flow rate was 1.2 ml/min. The sample injection volume was 5 μl. The detective wavelength was 205 nm. Results The calibration curve of matrine showed good linear response ranged from 54.50 to 872.00 μg/ml with r=0.999 1. The average recovery of spiked samples for matrine was 99.82% while the relative standard deviation for repetitions was 1.12%. Conclusion The method was simple, reliable and repeatable, which could be used for the quantitative determination of matrine of Lefu oral liquid.

Determination of matrine in Lefu oral liquid by HPLC

1. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China;Yichun University, Yichun 336000, China

2. Yichun University, Yichun 336000, China

3. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China

Received Date: 2015-02-04

Rev Recd Date: 2015-10-20

Key words:

HPLC /
matrine /
Lefu oral liquid

Abstract: Objective To establish a high performance liquid chromatography method for the determination of matrine in Lefu oral liquid. Methods The HPLC method was performed on a Diamonsil Platisil NH₂ column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-isopropyl alcohol-3% phosphoric acid solution (84:4:12). The flow rate was 1.2 ml/min. The sample injection volume was 5 μl. The detective wavelength was 205 nm. Results The calibration curve of matrine showed good linear response ranged from 54.50 to 872.00 μg/ml with r=0.999 1. The average recovery of spiked samples for matrine was 99.82% while the relative standard deviation for repetitions was 1.12%. Conclusion The method was simple, reliable and repeatable, which could be used for the quantitative determination of matrine of Lefu oral liquid.

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Reference (14)

[1]	陈征宇,孔爱英.军队医疗机构制剂标准提高计划实施构想[J].中国药事,2013,27(11):1157-1160.
[2]	汤迎爽,李富军.浅谈军队医院制剂发展方向[J].中医药导报,2012,18(7):120-121.
[3]	周苏萍,夏晓东,傅小英.军队药品制剂抽验质量分析与建议[J].解放军药学学报, 2010,26(2):176-177.
[4]	林厚文,廖万清,郭澄.乐肤口服液制备及临床应用[J].时珍国医国药,2001,12(3):221.
[5]	高学敏.中药学[M].北京:中国中医药出版社,2007:103.
[6]	Wu XN, Yamashita F, Hashida M,et al. Determination of matrine in rat plasma by high-performance liquid chromatography and its application to pharmacokinetic studies[J].Talanta,2003,59(5):965-971.
[7]	张平刚,沈亮,甘凤琼,等.高效液相色谱法测定越南槐不同生长季节及不同部位的苦参碱和氧化苦参碱的含量[J].时珍国医国药,2013,24(1):140-141.
[8]	余志华,王秀梅,吴雅莉,等.反相高效液相色谱法同时测定阴炎净洗液中苦参碱和氧化苦参碱含量[J]. 中国药业,2013,22(19):33-35.
[9]	于海红,姜音,丛景香,等.苦参粗提液中苦参碱和氧化苦参碱的高效液相色谱法测定[J].时珍国医国药,2013,24(1):126-127.
[10]	屈蓉,尹萌,张厚宝.高效液相色谱法测定止痢片中苦参碱的含量[J].时珍国医国药, 2008,19(11):2786-2787.
[11]	莫海玲,农英高,周佳宁.阴洁肤净洗液的质量标准研究[J].时珍国医国药,2007,18(3):632-633.
[12]	李智勇,孙冬梅,王洛临.痒宁颗粒的质量标准研究[J].时珍国医国药,2011,22(11):100-101.
[13]	Zhang L, Liu WT, Zhang RW, et al. Pharmacokinetic study of matrine, oxymatrine and oxysophocaroine in rat plasma after oral administration of Sophoraf lavescens Ait. Extract by liquid chromatography tandem mass spectrometry[J]. J Pharmaceut Biomed,2008,47(4-5):892-898.
[14]	国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社,2010:25-26,188-189.

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Determination of matrine in Lefu oral liquid by HPLC

doi: 10.3969/j.issn.1006-0111.2016.05.016

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