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ZHANG Jianhua, XU Yueming, HE Yuqiong, SONG Hongtao, DU Juan, ZHANG Qiaoyan. Studies on content determination and extraction method of iridoid glycosides in Morinda of ficinalis How[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 328-333. doi: 10.3969/j.issn.1006-0111.2017.04.010
Citation: CHENG Dan, CHEN Jianming. Simultaneous determination of paclitaxel-stearate (PTX-SA)and its bioactive metabolite paclitaxel (PTX)by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 224-227,273. doi: 10.3969/j.issn.1006-0111.2018.03.007

Simultaneous determination of paclitaxel-stearate (PTX-SA)and its bioactive metabolite paclitaxel (PTX)by RP-HPLC

doi: 10.3969/j.issn.1006-0111.2018.03.007
  • Received Date: 2017-12-18
  • Rev Recd Date: 2018-02-28
  • Objective To establish a reversed phase high performance liquid chromatography method which couldb be applied to determine the content of paclitaxel stearate(PTX-SA)and its bioactive metabolites paclitaxel(PTX)simultaneously. Methods Diazepam was chosen to be the internal standard and liquid-liquid extraction method was used for the preparation of sample solution with methyl tert-butyl ether. The two compounds were separated on Agilent Eclipse plus C18 column(4.6 mm×250 mm,5 μm) with gradient elution by using mobile phase of acetonitrile(A)-methanol(B)-water(C), the detection wavelength was 227 nm and the column temperature was 30℃. The flow rate was 1 ml/min and the injection volume was 20 μl. Results The standard curve exhibited good linearity within the concentration of PTX-SA from 0.5-250 μg/ml (r=0.997 6) and PTX from 0.05-25 μg/ml (r=0.997 9), meanwhile the LOQ of PTX-SA and PTX were 0.15 and 0.05 μg/ml, respectively. The recoveries were both larger than 90% with RSD less than 5%. Moreover, the RSD of intra-day and inter-day assays were both less than 5%. Conclusion The method was simple, effective, stable and accurate which could be suitable for the determination of PTX-SA and PTX in pharmacokinetic study.
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    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 22.9 %其他: 22.9 %其他: 2.2 %其他: 2.2 %Saitama: 0.5 %Saitama: 0.5 %上海: 3.5 %上海: 3.5 %北京: 19.2 %北京: 19.2 %巴音郭楞: 0.2 %巴音郭楞: 0.2 %广州: 0.5 %广州: 0.5 %成都: 0.5 %成都: 0.5 %杭州: 1.7 %杭州: 1.7 %深圳: 0.5 %深圳: 0.5 %芒廷维尤: 25.2 %芒廷维尤: 25.2 %西宁: 20.4 %西宁: 20.4 %达尔斯: 0.2 %达尔斯: 0.2 %运城: 0.2 %运城: 0.2 %郑州: 1.0 %郑州: 1.0 %长治: 0.2 %长治: 0.2 %黄冈: 0.7 %黄冈: 0.7 %其他其他Saitama上海北京巴音郭楞广州成都杭州深圳芒廷维尤西宁达尔斯运城郑州长治黄冈Highcharts.com
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Simultaneous determination of paclitaxel-stearate (PTX-SA)and its bioactive metabolite paclitaxel (PTX)by RP-HPLC

doi: 10.3969/j.issn.1006-0111.2018.03.007

Abstract: Objective To establish a reversed phase high performance liquid chromatography method which couldb be applied to determine the content of paclitaxel stearate(PTX-SA)and its bioactive metabolites paclitaxel(PTX)simultaneously. Methods Diazepam was chosen to be the internal standard and liquid-liquid extraction method was used for the preparation of sample solution with methyl tert-butyl ether. The two compounds were separated on Agilent Eclipse plus C18 column(4.6 mm×250 mm,5 μm) with gradient elution by using mobile phase of acetonitrile(A)-methanol(B)-water(C), the detection wavelength was 227 nm and the column temperature was 30℃. The flow rate was 1 ml/min and the injection volume was 20 μl. Results The standard curve exhibited good linearity within the concentration of PTX-SA from 0.5-250 μg/ml (r=0.997 6) and PTX from 0.05-25 μg/ml (r=0.997 9), meanwhile the LOQ of PTX-SA and PTX were 0.15 and 0.05 μg/ml, respectively. The recoveries were both larger than 90% with RSD less than 5%. Moreover, the RSD of intra-day and inter-day assays were both less than 5%. Conclusion The method was simple, effective, stable and accurate which could be suitable for the determination of PTX-SA and PTX in pharmacokinetic study.

ZHANG Jianhua, XU Yueming, HE Yuqiong, SONG Hongtao, DU Juan, ZHANG Qiaoyan. Studies on content determination and extraction method of iridoid glycosides in Morinda of ficinalis How[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(4): 328-333. doi: 10.3969/j.issn.1006-0111.2017.04.010
Citation: CHENG Dan, CHEN Jianming. Simultaneous determination of paclitaxel-stearate (PTX-SA)and its bioactive metabolite paclitaxel (PTX)by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 224-227,273. doi: 10.3969/j.issn.1006-0111.2018.03.007
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