Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 36 Issue 3
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2018  >  36(3): 228-233

HE Maotao, SONG Li, WEN Xia, LIU Lan. The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 228-233. doi: 10.3969/j.issn.1006-0111.2018.03.008
Citation: HE Maotao, SONG Li, WEN Xia, LIU Lan. The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 228-233. doi: 10.3969/j.issn.1006-0111.2018.03.008
shu

The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC

doi: 10.3969/j.issn.1006-0111.2018.03.008

  • School of Pharmacy, Chengdu College of Medicine, Chengdu 610500, China

  • Received Date: 2017-07-11
  • Rev Recd Date: 2018-01-20
  • Objective To observe the effect of salted processing on the contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma. Methods UV was used for the determination of total alismol's content in crude Alisma Rhizoma and salted Alisma Rhizoma with Meta Nitrobenzene and Potassium Hydroxide as the developers. The detection wavelength was 545 nm. HPLC was used for the determination of 24-acetylalisol A's and 23-acetylalisol B's contents in crude Alisma Rhizoma and salted Alisma Rhizoma with Elite C18 (4.6 mm×250 mm, 5 μm) as the chromatographic column. The mobile phase was gradient elution with acetonitrile and water. The detection wavelength was 208 nm. The column temperature was set at 25℃. The flow rate was 1 ml/min. Results The linear relationships of total alismol, 24-acetylalisol A and 23-acetylalisol B were fine (r>0.999 0) in the linear ranges by UV and HPLC. The average recoveries of them in crude Alisma Rhizoma and salted Alisma Rhizoma ranged from 97.81%-100.05%,with the RSD less than 3%. The average contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in ten batches of crude Alisma Rhizoma were 4.41%, 0.208%, 0.065% respectively. The average contents of them in ten batches of salted Alisma Rhizoma were 9.62%, 0.232%, 0.017% respectively. Conclusion The contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma were influenced considerably by salted processing. The content of total alismol increased obviously. The content of 24-acetylalisol A increased slightly. The content of 23-acetylalisol B decreased obviously.
  • [1] 国家药典委员会. 中华人民共和国药典(一部):2015年版[S]. 北京:中国医药科技出版社,2015:229.
    [2] 田婷,陈华,林瑞超,等. 泽泻药理与毒理作用的研究进展[J]. 中药材,2014,37(11):2103-2108.
    [3] 禹建春,叶红梅,林西西. 泽泻的药理研究概况[J]. 海峡药学,2011,23(2):92-93.
    [4] 陈利娟,李卿. 泽泻的研究进展[J]. 中国药业,2016,25(21):1-3.
    [5] 陈曦. 泽泻的研究现状与进展[J]. 中国民族民间医药,2011,9(3):50-53.
    [6] 胡雪艳,陈海霞,高文远,等. 泽泻化学成分的研究[J]. 中草药,2008,12:1788-1790.
    [7] 王建平,傅旭春. 泽泻化学成分和药理作用研究进展[J]. 浙江医学,2012,34(14):1235-1237.
    [8] 徐硕,夏路风,金鹏飞,等. 泽泻的化学成分及生物活性研究进展[J]. 中国医药导报,2015,12(27):47-51.
    [9] 李寅,陈岩松,马宝东. 泽泻炮制及副作用[J]. 实用中医内科杂志,2013,27(7):141-142.
    [10] 龚又明,高妮. 泽泻不同炮制品药理研究[J]. 新中医,2011(7):136-138.
    [11] 陈晓蕾,李红阳. 泽泻生品及不同炮制品对小鼠急性肝损伤的保护作用[J]. 中药材,2006,29(6):592-593.
    [12] 王巧娥,邱招钗,靳保辉,等. 泽泻中三萜醇酮类化合物总量的快速测定方法研究[J]. 厦门大学学报(自然科学版),2003,42(6):751-755.
    [13] 戴小欢,曲琰,贾天柱. 不同炮制方法对泽泻中主要成分含量的影响[J]. 吉林中医药,2009(4):30-33.
    [14] 韩伟健,林晓彤,郭娜,等. 泽泻盐制前后成分转化的1HNMR分析[J]. 波谱学杂志,2016(1):117-124.
    [15] 谢雪,许枬,张宏达. 泽泻盐炙过程中化学成分转化研究[C]. 中华中医药学会中药炮制分会2011年学术年会论文集,2011:7.
    [16] 郑云枫,朱玉岚,彭国平. 泽泻炮制过程中23-乙酰泽泻醇B的转化[J]. 中草药,2006,37(10):1479-1482.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(3410) PDF downloads(720) Cited by()

Related
Proportional views

The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC

doi: 10.3969/j.issn.1006-0111.2018.03.008
  • School of Pharmacy, Chengdu College of Medicine, Chengdu 610500, China
  • Received Date: 2017-07-11
  • Rev Recd Date: 2018-01-20

Abstract: Objective To observe the effect of salted processing on the contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma. Methods UV was used for the determination of total alismol's content in crude Alisma Rhizoma and salted Alisma Rhizoma with Meta Nitrobenzene and Potassium Hydroxide as the developers. The detection wavelength was 545 nm. HPLC was used for the determination of 24-acetylalisol A's and 23-acetylalisol B's contents in crude Alisma Rhizoma and salted Alisma Rhizoma with Elite C18 (4.6 mm×250 mm, 5 μm) as the chromatographic column. The mobile phase was gradient elution with acetonitrile and water. The detection wavelength was 208 nm. The column temperature was set at 25℃. The flow rate was 1 ml/min. Results The linear relationships of total alismol, 24-acetylalisol A and 23-acetylalisol B were fine (r>0.999 0) in the linear ranges by UV and HPLC. The average recoveries of them in crude Alisma Rhizoma and salted Alisma Rhizoma ranged from 97.81%-100.05%,with the RSD less than 3%. The average contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in ten batches of crude Alisma Rhizoma were 4.41%, 0.208%, 0.065% respectively. The average contents of them in ten batches of salted Alisma Rhizoma were 9.62%, 0.232%, 0.017% respectively. Conclusion The contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma were influenced considerably by salted processing. The content of total alismol increased obviously. The content of 24-acetylalisol A increased slightly. The content of 23-acetylalisol B decreased obviously.

HE Maotao, SONG Li, WEN Xia, LIU Lan. The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 228-233. doi: 10.3969/j.issn.1006-0111.2018.03.008
Citation: HE Maotao, SONG Li, WEN Xia, LIU Lan. The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 228-233. doi: 10.3969/j.issn.1006-0111.2018.03.008
shu

    HTML

Reference (16)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return