HE Maotao, SONG Li, WEN Xia, LIU Lan. The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 228-233. doi: 10.3969/j.issn.1006-0111.2018.03.008
| Citation:
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HE Maotao, SONG Li, WEN Xia, LIU Lan. The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 228-233. doi: 10.3969/j.issn.1006-0111.2018.03.008
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The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC
- Received Date: 2017-07-11
- Rev Recd Date:
2018-01-20
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Abstract
Objective To observe the effect of salted processing on the contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma. Methods UV was used for the determination of total alismol's content in crude Alisma Rhizoma and salted Alisma Rhizoma with Meta Nitrobenzene and Potassium Hydroxide as the developers. The detection wavelength was 545 nm. HPLC was used for the determination of 24-acetylalisol A's and 23-acetylalisol B's contents in crude Alisma Rhizoma and salted Alisma Rhizoma with Elite C18 (4.6 mm×250 mm, 5 μm) as the chromatographic column. The mobile phase was gradient elution with acetonitrile and water. The detection wavelength was 208 nm. The column temperature was set at 25℃. The flow rate was 1 ml/min. Results The linear relationships of total alismol, 24-acetylalisol A and 23-acetylalisol B were fine (r>0.999 0) in the linear ranges by UV and HPLC. The average recoveries of them in crude Alisma Rhizoma and salted Alisma Rhizoma ranged from 97.81%-100.05%,with the RSD less than 3%. The average contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in ten batches of crude Alisma Rhizoma were 4.41%, 0.208%, 0.065% respectively. The average contents of them in ten batches of salted Alisma Rhizoma were 9.62%, 0.232%, 0.017% respectively. Conclusion The contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma were influenced considerably by salted processing. The content of total alismol increased obviously. The content of 24-acetylalisol A increased slightly. The content of 23-acetylalisol B decreased obviously.
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