XIE Hua, WANG Yanling, WANG Chang, LI Wenbin, JIA Zhengping, WANG Rong. The protective effects of metoprolol on rat myocardium at 3 400 m[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 238-242. doi: 10.3969/j.issn.1006-0111.2018.03.010
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XIE Hua, WANG Yanling, WANG Chang, LI Wenbin, JIA Zhengping, WANG Rong. The protective effects of metoprolol on rat myocardium at 3 400 m[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 238-242. doi: 10.3969/j.issn.1006-0111.2018.03.010
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The protective effects of metoprolol on rat myocardium at 3 400 m
- Received Date: 2018-02-23
- Rev Recd Date:
2018-04-04
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Abstract
Objective To investigate the effects on myocardial mitochondria-related indexes in rats after rapid elevation to 3 400 m altitude and myocardial protection effects with metoprolol. Methods Male Wistar rats were randomly divided into three groups with 12 rats in each group as follows, group A (normal group, 55 m above sea level), group B (hypoxia group, 3 400 m above sea level), group C (metoprolol group, 3 400 m above sea level). Heart rate and blood pressure were measured during the rapid transfer from the plain (Shanghai 55 m) to plateau of 3 400 m. Then, the rats were anesthetized with chloral hydrate. The blood was collected from the abdominal aorta and blood gas indexes were measured. The heart tissue was homogenized. The oxidativee stress related parameters, such as superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) were assayed. The mitochondrial function related parameters, such as complexes-Ⅰ, Ⅱ, Ⅲ, Ⅳ, succinate dehydrogenase (SDH), malate dehydrogenase (MDH) and adenosine triphosphate (ATP) were measured. Results After the rats were exposed to high altitude, the blood oxygen tension and oxygen saturation in the metoprolol group were increased in comparison with the hypoxia group. Compared with the normal group, the content of MDA in the hypoxia group was increased significantly, while the content of SOD and NO were decreased significantly (P<0.01), the mitochondrial complex Ⅰ, Ⅱ, Ⅲ and Ⅳ were also decreased 44.49%, 32.38%, 34.43% and 20.16%. Compared with hypoxia group, the MDA was decreased by 13.72%, while SOD and NO were increased by 25.73% and 168.55%, the activity of mitochondrial complexes were also increased by 44.92%, 33.73%, 37.11% and 10.09% for metoprolol group. The respiratory chain SDH and MDH in hypoxic group were decreased by 29.51% and 42.79% and the activity of Na+-K+-ATPase, Ca2+-Mg2+-ATPase and T-ATPase were decreased by 20.24%, 27.56% and 33.32% in comparison with the normal group. After intervention with metoprolol, SDH and MDH were increased by 47.36% and 88.57% separately, the activities of Na+-K+-ATPase, Ca2+-Mg2+-ATPase and T-ATPase were also increased by 31.92%, 26.95%, 44.93% in comparison with hypoxia group. Those results indicated that metoprolol can increase ATPase activity. Conclusion Under the condition of acute hypoxia at 3 400 m above sea level, myocardial injury is related to the changes of oxidative stress and mitochondrial-related enzyme activity. Metoprolol helps to normalize the related indicators and provides the protection for myocardial injury caused by hypoxia.
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