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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2018  >  36(3): 250-254

WU Ningzi, MA HuiPing, WANG Xin, HE Lei, JING Linlin, JIA ZhengPing. Study on myocardium and brain damage in rats by simulating high altitude[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 250-254. doi: 10.3969/j.issn.1006-0111.2018.03.013
Citation: WU Ningzi, MA HuiPing, WANG Xin, HE Lei, JING Linlin, JIA ZhengPing. Study on myocardium and brain damage in rats by simulating high altitude[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 250-254. doi: 10.3969/j.issn.1006-0111.2018.03.013
shu

Study on myocardium and brain damage in rats by simulating high altitude

doi: 10.3969/j.issn.1006-0111.2018.03.013
  • 1.

    School of Pharmacy, Lanzhou University, Lanzhou 730000, China;Lanzhou General Hospital Key Lab of PLA for Prevention and Treatment of Injuries induced by High Altitude, Lanzhou 730050, China

  • 2.

    Lanzhou General Hospital Key Lab of PLA for Prevention and Treatment of Injuries induced by High Altitude, Lanzhou 730050, China

  • Received Date: 2017-09-14
  • Rev Recd Date: 2018-01-24
  • Objective To investigate the effect of hypoxia with cold on the heart and brain damage in rats by simulating 6 000 m high altitude at different exposure time, established a rat model of acute mountain sickness for the related mechanism studies. Methods 32 healthy male Wistar rats were randomly divided into normal control group, hypoxia with cold 1 d, 3 d and 5 d group, 8 rats in each group. The normal control group was kept in the plain environment (1 500 m) without any treatment. The other three groups were placed in large hypobaric hypoxia chamber to simulate 6 000 m altitude with different exposed times. HE staining was used to observe the pathological changes of heart and brain tissue. The changes of biochemical indexes were measured to evaluate the damage of heart and brain tissue at different hypoxia times. Results HE staining showed that hypoxia with cold induced rat heart and brain damage with different degrees. The myocardial tissue damage was increased with exposure time. The most serious brain damage happened in day 3. Compared with the normal control group, the content of MDA and LD in the myocardial tissue of hypoxia rats were significantly increased (P<0.05 or P<0.01) with prolonged time, while the contents of GSH, T-SOD and the activity of Na+ K+-ATPase were reduced (P<0.05 or P<0.01). The content of MDA in brain tissue was significantly increased at day 1 and day 3 (P<0.05 or P<0.01). LD content was significantly increased (P<0.05) with time. The content of GSH, the activity of T-SOD and Na+ K+-ATPase were significantly reduced in day 3 (P<0.05). Conclusion Simulating an altitude of 6 000 m caused obvious damage on the heart and brain tissues of rats. The degree of damage was related to the exposure time to hypoxia with cold. The decrease of body's antioxidant capacity, the increase of free radicals and energy metabolism disorders are important factors leading to heart and brain injury.
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Study on myocardium and brain damage in rats by simulating high altitude

doi: 10.3969/j.issn.1006-0111.2018.03.013
  • 1. School of Pharmacy, Lanzhou University, Lanzhou 730000, China;Lanzhou General Hospital Key Lab of PLA for Prevention and Treatment of Injuries induced by High Altitude, Lanzhou 730050, China
  • 2. Lanzhou General Hospital Key Lab of PLA for Prevention and Treatment of Injuries induced by High Altitude, Lanzhou 730050, China
  • Received Date: 2017-09-14
  • Rev Recd Date: 2018-01-24

Abstract: Objective To investigate the effect of hypoxia with cold on the heart and brain damage in rats by simulating 6 000 m high altitude at different exposure time, established a rat model of acute mountain sickness for the related mechanism studies. Methods 32 healthy male Wistar rats were randomly divided into normal control group, hypoxia with cold 1 d, 3 d and 5 d group, 8 rats in each group. The normal control group was kept in the plain environment (1 500 m) without any treatment. The other three groups were placed in large hypobaric hypoxia chamber to simulate 6 000 m altitude with different exposed times. HE staining was used to observe the pathological changes of heart and brain tissue. The changes of biochemical indexes were measured to evaluate the damage of heart and brain tissue at different hypoxia times. Results HE staining showed that hypoxia with cold induced rat heart and brain damage with different degrees. The myocardial tissue damage was increased with exposure time. The most serious brain damage happened in day 3. Compared with the normal control group, the content of MDA and LD in the myocardial tissue of hypoxia rats were significantly increased (P<0.05 or P<0.01) with prolonged time, while the contents of GSH, T-SOD and the activity of Na+ K+-ATPase were reduced (P<0.05 or P<0.01). The content of MDA in brain tissue was significantly increased at day 1 and day 3 (P<0.05 or P<0.01). LD content was significantly increased (P<0.05) with time. The content of GSH, the activity of T-SOD and Na+ K+-ATPase were significantly reduced in day 3 (P<0.05). Conclusion Simulating an altitude of 6 000 m caused obvious damage on the heart and brain tissues of rats. The degree of damage was related to the exposure time to hypoxia with cold. The decrease of body's antioxidant capacity, the increase of free radicals and energy metabolism disorders are important factors leading to heart and brain injury.

WU Ningzi, MA HuiPing, WANG Xin, HE Lei, JING Linlin, JIA ZhengPing. Study on myocardium and brain damage in rats by simulating high altitude[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 250-254. doi: 10.3969/j.issn.1006-0111.2018.03.013
Citation: WU Ningzi, MA HuiPing, WANG Xin, HE Lei, JING Linlin, JIA ZhengPing. Study on myocardium and brain damage in rats by simulating high altitude[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 250-254. doi: 10.3969/j.issn.1006-0111.2018.03.013
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