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SUN Yuhan, XU Ziyi, LIAO Jun, ZHANG He, FAN Li, LU Ying. Content measurement of doxorubicin hydrochloride and lonidamine by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(3): 127-130. doi: 10.12206/j.issn.2097-2024.202306043
Citation:
MA Huiping, ZHANG Jun, JIA Zhengping, MENG Panpan, JING Linlin, WANG Rong. Anti-hypoxia activity and its protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 255-259. doi: 10.3969/j.issn.1006-0111.2018.03.014
Anti-hypoxia activity and its protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia
Lanzhou General Hospital Key Lab of PLA for Prevention and Treatment of Injuries induced by High Altitude, Lanzhou 730050, China
Received Date: 2017-09-14
Rev Recd Date:
2018-01-24
Abstract
Objective To investigate anti-hypoxia activity and protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia. Methods The anti-hypoxic activity of Lishukang capsule was evaluated with normobaric hypoxia test and acute hypobaric hypoxia test in mice. In addition, rats were exposed to large hypobaric hypoxia chamber stimulating 8 000 m altitude. The pathological changes of rat brain tissue before and after hypoxia were observed. The oxidative stress indicators and metabolism parameters in brain were measured. Results The low, medium and high Lishukang doses can effectively prolong the survival time of mice (P<0.01) in the dose dependent manner. The medium and high Lishukang doses were significantly better than those of Rhodiola rosea capsules (P<0.05 or P<0.01). The low, medium, high Lishukang dose groups reduced the mortality of acute hypobaric hypoxia mice (P<0.01) with dose dependent effects. The mice mortality in medium and high dose groups was lower than that of Rhodiolarosea group (P<0.01). Compared with normal control group, the hypoxic model rats exhibited pathological injury in the brain tissue after exposure to hypobaric hypoxia stimulating 8 000 m altitude. The contents of MDA, H2O2, NO, LD and LDH activity increased significantly (P<0.05 or P<0.01), while the activities of SOD, CAT, GPX were significantly decreased (P<0.05 or P<0.01). After pretreatment with Lishukang capsule, the pathological damage of rat brain tissue was alleviated and the content of MDA, NO in the brain tissue was reduced (P<0.05 or P<0.01). The levels of H2O2, LD content and LDH activity in medium and high dose groups were significantly decreased (P<0.05 or P<0.01). The activities of SOD, CAT and GPX in high dose group were significantly increased (P<0.05). Conclusion Lishukang capsule has good anti-hypoxia activity. It provides protective effect for the injuries induced by hypobaric hypoxia in rats. The mechanism may related to the improvement of antioxidant capability, reduction of free radical damage and amelioration of energy metabolism.
Ma HP,Fan PC,Jing LL,et al. Anti-hypoxic activity at simulated high altitude was isolated in petroleum ether extract of Saussurea involucrata[J]. J. Ethnopharmacol, 2011, 137(3):1510-1515.
[3]
Maiti P, Singh SB, Sharma AK, et al. Hypobaric hypoxia induces oxidative stress in rat brain[J]. Neurochem Int,2006,49(8):709-716.
Maity C, Lahiri P, Adak A, et al. Hypobaric-hypoxia induces alteration in microbes and microbes-associated enzyme profile in rat colonic samples[J]. Biomed Environ Sci,2013,26(10):869-873.
Qian J, Jiang F,Wang B, et al. Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells[J]. J Ethnopharmacol, 2010, 128(2):438-445.
[8]
Shen ZB,Yin YQ,Tang CP, et al. Pharmacodynamic screening and simulation study of anti-hypoxia active fraction of Xiangdan injection[J]. J Ethnopharmacol, 2010,127(1):103-107.
[9]
Bailey DM,Bärtsch P, Knauth M, et al. Emerging concepts in acute mountain sickness and high-altitude cerebral edema:from the molecular to the morphological[J]. Cell Mol Life Sci, 2009, 66(22):3583-3594.
Abstract: Objective To investigate anti-hypoxia activity and protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia. Methods The anti-hypoxic activity of Lishukang capsule was evaluated with normobaric hypoxia test and acute hypobaric hypoxia test in mice. In addition, rats were exposed to large hypobaric hypoxia chamber stimulating 8 000 m altitude. The pathological changes of rat brain tissue before and after hypoxia were observed. The oxidative stress indicators and metabolism parameters in brain were measured. Results The low, medium and high Lishukang doses can effectively prolong the survival time of mice (P<0.01) in the dose dependent manner. The medium and high Lishukang doses were significantly better than those of Rhodiola rosea capsules (P<0.05 or P<0.01). The low, medium, high Lishukang dose groups reduced the mortality of acute hypobaric hypoxia mice (P<0.01) with dose dependent effects. The mice mortality in medium and high dose groups was lower than that of Rhodiolarosea group (P<0.01). Compared with normal control group, the hypoxic model rats exhibited pathological injury in the brain tissue after exposure to hypobaric hypoxia stimulating 8 000 m altitude. The contents of MDA, H2O2, NO, LD and LDH activity increased significantly (P<0.05 or P<0.01), while the activities of SOD, CAT, GPX were significantly decreased (P<0.05 or P<0.01). After pretreatment with Lishukang capsule, the pathological damage of rat brain tissue was alleviated and the content of MDA, NO in the brain tissue was reduced (P<0.05 or P<0.01). The levels of H2O2, LD content and LDH activity in medium and high dose groups were significantly decreased (P<0.05 or P<0.01). The activities of SOD, CAT and GPX in high dose group were significantly increased (P<0.05). Conclusion Lishukang capsule has good anti-hypoxia activity. It provides protective effect for the injuries induced by hypobaric hypoxia in rats. The mechanism may related to the improvement of antioxidant capability, reduction of free radical damage and amelioration of energy metabolism.
SUN Yuhan, XU Ziyi, LIAO Jun, ZHANG He, FAN Li, LU Ying. Content measurement of doxorubicin hydrochloride and lonidamine by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(3): 127-130. doi: 10.12206/j.issn.2097-2024.202306043
Citation:
MA Huiping, ZHANG Jun, JIA Zhengping, MENG Panpan, JING Linlin, WANG Rong. Anti-hypoxia activity and its protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 255-259. doi: 10.3969/j.issn.1006-0111.2018.03.014
Ma HP,Fan PC,Jing LL,et al. Anti-hypoxic activity at simulated high altitude was isolated in petroleum ether extract of Saussurea involucrata[J]. J. Ethnopharmacol, 2011, 137(3):1510-1515.
[3]
Maiti P, Singh SB, Sharma AK, et al. Hypobaric hypoxia induces oxidative stress in rat brain[J]. Neurochem Int,2006,49(8):709-716.
Maity C, Lahiri P, Adak A, et al. Hypobaric-hypoxia induces alteration in microbes and microbes-associated enzyme profile in rat colonic samples[J]. Biomed Environ Sci,2013,26(10):869-873.
Qian J, Jiang F,Wang B, et al. Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells[J]. J Ethnopharmacol, 2010, 128(2):438-445.
[8]
Shen ZB,Yin YQ,Tang CP, et al. Pharmacodynamic screening and simulation study of anti-hypoxia active fraction of Xiangdan injection[J]. J Ethnopharmacol, 2010,127(1):103-107.
[9]
Bailey DM,Bärtsch P, Knauth M, et al. Emerging concepts in acute mountain sickness and high-altitude cerebral edema:from the molecular to the morphological[J]. Cell Mol Life Sci, 2009, 66(22):3583-3594.
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SUN Yuhan, XU Ziyi, LIAO Jun, ZHANG He, FAN Li, LU Ying. Content measurement of doxorubicin hydrochloride and lonidamine by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(3): 127-130. doi: 10.12206/j.issn.2097-2024.202306043
SUN Yuhan, XU Ziyi, LIAO Jun, ZHANG He, FAN Li, LU Ying. Content measurement of doxorubicin hydrochloride and lonidamine by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(3): 127-130. doi: 10.12206/j.issn.2097-2024.202306043
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