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XU Ziyi, SUN Yuhan, FAN Li, LU Guangzhao, ZHANG Yingnan, ZHANG He. Preparation and cytotoxicity of doxorubicin-containing gold nanoparticles[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(2): 73-77, 81. doi: 10.12206/j.issn.2097-2024.202308043
Citation: WANG Yan, SHU Peng, SHEN Li, DING ming, HU Zhenlin, WU Mingjiang. Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(3): 231-236. doi: 10.3969/j.issn.1006-0111.2019.03.008

Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract

doi: 10.3969/j.issn.1006-0111.2019.03.008
  • Received Date: 2018-10-08
  • Rev Recd Date: 2018-11-26
  • Objective To investigate the anti-oxidative effects of the Schisandra chinensis extract in skin cells and develop the application as skin anti-oxidant. Methods The ethanol extraction process of Schisandra chinensis was optimized with hot water pre-treatment and steam distillation to improve the color and odor of the extract.The tert-butyl hydroperoxide (tBHP)-induced cell oxidative injured model in HaCaT keratinocytes was used as a cellular model to study the protective effects of the extract.The cell viability was assayed with CCK-8 kit.Cell apoptosis was detected with flow cytometry by AnnexinV-FITC and PI staining.The intracellular level of reactive oxygen species (ROS) was measured by 2,7-dichlorodi-hydrofluorescein diacetate (DCFH-DA) assay. Results The Schisandra extract prepared by optimized extraction technology exhibited significant improvements in color and odor.The HaCaT cells pretreated with Schisandra extract effectively reduced the cell apoptosis induced by tBHP.The extract increased the survival rate of HaCaT cells and decreased intracellular ROS level. Conclusion The extract with optimized technology improved the color and odor.Its antioxidant activity was still maintained.It could be developed into a novel skin protectant against oxidative damage.
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  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-04020406080Highcharts.com
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    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 47.8 %其他: 47.8 %其他: 0.4 %其他: 0.4 %Central District: 0.2 %Central District: 0.2 %San Jose: 0.1 %San Jose: 0.1 %上海: 3.1 %上海: 3.1 %东莞: 2.3 %东莞: 2.3 %临沂: 0.1 %临沂: 0.1 %丽水: 0.1 %丽水: 0.1 %乌鲁木齐: 0.2 %乌鲁木齐: 0.2 %保定: 0.2 %保定: 0.2 %儋州: 0.1 %儋州: 0.1 %兰州: 0.1 %兰州: 0.1 %北京: 8.8 %北京: 8.8 %十堰: 0.1 %十堰: 0.1 %南京: 1.3 %南京: 1.3 %南宁: 0.7 %南宁: 0.7 %南昌: 0.1 %南昌: 0.1 %厦门: 0.1 %厦门: 0.1 %古吉拉特: 0.1 %古吉拉特: 0.1 %合肥: 1.4 %合肥: 1.4 %吉林: 0.1 %吉林: 0.1 %呼和浩特: 0.2 %呼和浩特: 0.2 %咸阳: 0.1 %咸阳: 0.1 %哈尔滨: 0.4 %哈尔滨: 0.4 %唐山: 0.1 %唐山: 0.1 %商丘: 0.1 %商丘: 0.1 %大庆: 0.1 %大庆: 0.1 %大连: 0.4 %大连: 0.4 %天津: 1.2 %天津: 1.2 %太原: 0.5 %太原: 0.5 %威海: 0.1 %威海: 0.1 %宁波: 0.3 %宁波: 0.3 %宜春: 0.2 %宜春: 0.2 %宣城: 0.2 %宣城: 0.2 %宿迁: 0.1 %宿迁: 0.1 %常州: 0.1 %常州: 0.1 %平顶山: 0.1 %平顶山: 0.1 %广州: 1.5 %广州: 1.5 %开封: 0.2 %开封: 0.2 %张家口: 0.1 %张家口: 0.1 %张家界: 0.1 %张家界: 0.1 %徐州: 0.4 %徐州: 0.4 %恩施: 0.1 %恩施: 0.1 %成都: 0.7 %成都: 0.7 %昆明: 0.9 %昆明: 0.9 %晋中: 0.1 %晋中: 0.1 %朝阳: 0.3 %朝阳: 0.3 %本溪: 0.1 %本溪: 0.1 %杭州: 1.3 %杭州: 1.3 %桂林: 0.2 %桂林: 0.2 %武汉: 1.2 %武汉: 1.2 %汕头: 0.1 %汕头: 0.1 %江门: 0.3 %江门: 0.3 %沈阳: 0.5 %沈阳: 0.5 %泰安: 0.1 %泰安: 0.1 %洛阳: 0.5 %洛阳: 0.5 %济南: 0.4 %济南: 0.4 %海口: 0.3 %海口: 0.3 %淮北: 0.1 %淮北: 0.1 %淮安: 0.1 %淮安: 0.1 %深圳: 0.8 %深圳: 0.8 %温州: 0.2 %温州: 0.2 %滨州: 0.1 %滨州: 0.1 %潍坊: 0.6 %潍坊: 0.6 %澳门: 0.1 %澳门: 0.1 %石家庄: 1.0 %石家庄: 1.0 %福州: 0.4 %福州: 0.4 %秦皇岛: 0.4 %秦皇岛: 0.4 %绍兴: 0.1 %绍兴: 0.1 %芒廷维尤: 2.3 %芒廷维尤: 2.3 %芜湖: 0.2 %芜湖: 0.2 %芝加哥: 0.4 %芝加哥: 0.4 %苏州: 0.2 %苏州: 0.2 %葫芦岛: 0.4 %葫芦岛: 0.4 %蚌埠: 0.1 %蚌埠: 0.1 %衡水: 0.4 %衡水: 0.4 %衡阳: 0.1 %衡阳: 0.1 %西宁: 1.6 %西宁: 1.6 %西安: 0.5 %西安: 0.5 %贵阳: 0.6 %贵阳: 0.6 %达州: 0.1 %达州: 0.1 %运城: 0.4 %运城: 0.4 %通辽: 0.1 %通辽: 0.1 %遵义: 0.2 %遵义: 0.2 %郑州: 0.9 %郑州: 0.9 %重庆: 0.9 %重庆: 0.9 %金华: 0.2 %金华: 0.2 %镇江: 0.2 %镇江: 0.2 %长春: 0.1 %长春: 0.1 %长沙: 4.1 %长沙: 4.1 %青岛: 1.2 %青岛: 1.2 %马鞍山: 0.1 %马鞍山: 0.1 %黄冈: 0.2 %黄冈: 0.2 %黄石: 0.1 %黄石: 0.1 %黔南: 0.3 %黔南: 0.3 %其他其他Central DistrictSan Jose上海东莞临沂丽水乌鲁木齐保定儋州兰州北京十堰南京南宁南昌厦门古吉拉特合肥吉林呼和浩特咸阳哈尔滨唐山商丘大庆大连天津太原威海宁波宜春宣城宿迁常州平顶山广州开封张家口张家界徐州恩施成都昆明晋中朝阳本溪杭州桂林武汉汕头江门沈阳泰安洛阳济南海口淮北淮安深圳温州滨州潍坊澳门石家庄福州秦皇岛绍兴芒廷维尤芜湖芝加哥苏州葫芦岛蚌埠衡水衡阳西宁西安贵阳达州运城通辽遵义郑州重庆金华镇江长春长沙青岛马鞍山黄冈黄石黔南Highcharts.com
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Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract

doi: 10.3969/j.issn.1006-0111.2019.03.008

Abstract: Objective To investigate the anti-oxidative effects of the Schisandra chinensis extract in skin cells and develop the application as skin anti-oxidant. Methods The ethanol extraction process of Schisandra chinensis was optimized with hot water pre-treatment and steam distillation to improve the color and odor of the extract.The tert-butyl hydroperoxide (tBHP)-induced cell oxidative injured model in HaCaT keratinocytes was used as a cellular model to study the protective effects of the extract.The cell viability was assayed with CCK-8 kit.Cell apoptosis was detected with flow cytometry by AnnexinV-FITC and PI staining.The intracellular level of reactive oxygen species (ROS) was measured by 2,7-dichlorodi-hydrofluorescein diacetate (DCFH-DA) assay. Results The Schisandra extract prepared by optimized extraction technology exhibited significant improvements in color and odor.The HaCaT cells pretreated with Schisandra extract effectively reduced the cell apoptosis induced by tBHP.The extract increased the survival rate of HaCaT cells and decreased intracellular ROS level. Conclusion The extract with optimized technology improved the color and odor.Its antioxidant activity was still maintained.It could be developed into a novel skin protectant against oxidative damage.

XU Ziyi, SUN Yuhan, FAN Li, LU Guangzhao, ZHANG Yingnan, ZHANG He. Preparation and cytotoxicity of doxorubicin-containing gold nanoparticles[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(2): 73-77, 81. doi: 10.12206/j.issn.2097-2024.202308043
Citation: WANG Yan, SHU Peng, SHEN Li, DING ming, HU Zhenlin, WU Mingjiang. Study on optimized extraction of Schisandra chinensis and cutaneous cell antioxidative effect of its extract[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(3): 231-236. doi: 10.3969/j.issn.1006-0111.2019.03.008
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