中药蝼蛄的高效液相指纹图谱研究
Studies on HPLC fingerprint spectrum of Gryllotalpa
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摘要: 目的 建立中药蝼蛄及其伪品的HPLC指纹图谱鉴别方法.方法 两种蝼蛄(东方蝼蛄Gryllotalpa orientalis Burmeister和华北蝼蛄G.unipina Saussure)及其伪品(蟋蟀Teleogryllus emus)的水溶液采用高效液相色谱法分析,流动相甲醇-水=10:90,检测波长260 nm.结果 HPLC图谱显示2个蝼蛄品种和混乱品种蟋蟀具有7个共有峰,并且具有各自的指纹特征.结论 可利用指纹图谱的差异进行中药蝼蛄品种以及混乱品种间的鉴定.Abstract: Objective To establish HPLC fingerprint spectrum of commercial species of Gryllotalpa(Gryllotalpa orientalis Burmeister, G.unipina Saussure) and their false species(Teleogryllus emus). Methods Samples extracted with water by heating and refluence were analized by HPLC. HPLC mobile phase consisted of methanol-water(10:90) and detect wavelength was set at 260nm. Results Two species of Gryllotalpa and false variety had 7 common peaks, and every species had it's own characteristic peaks. Conclusion Varieties and confusion variety could be identified with differences in fingerprint by HPLC.
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Key words:
- Gryllotalpa /
- HPLC /
- Fingerprint spectrum
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