反相高效液相色谱法测定芩蓝滴鼻液中黄芩苷含量
Quantitative analysis of baicalin in Qinlan nasal drops by RP-HPLC
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摘要: 目的建立反相高效液相色谱法分离测定芩蓝滴鼻液中黄芩苷的含量。方法:采用Gemini C18
110A(4.60 mm×250 mm,5μm)色谱柱;流动相为甲醇-0.2%磷酸溶液(50:50);流速为0.8 ml/min;检测波长为277 nm;进样量10μl。结果黄芩苷浓度在709.4~7094.0μg/ml范围内与峰面积呈良好的线性关系,r=0.999 9;平均回收率为99.6%,RSD为1.33%(n=9)。结论本方法简便、准确、专属性强,可用于测定芩蓝滴鼻液中黄芩苷的含量。Abstract: Objective To develop RP-HPLC method for the determination of baicalin in Qinlan nasal drops. Methods The chromatographic analysis was performed on Gemini C18 110A(4.60 mm×250 mm,5 μm)column.The mobile phase was consisted of methanol-0.2% phosphoric acid solution(50: 50).The flow rate was 0.8 ml/min.The detection wavelength was set at 277 nm,with injection volume 10 μl. Results A good linearity was obtained over the range of 709.4～7094.0μg/ml(r=0.999 9).The average recovery of baicalin was 99.6%,with RSD 1.33%(n=9). Conclusion The method is simple,accurate,specific and will be used for the quality control of Qinlan nasal drops.-
Key words:
- RP-HPLC /
- Qinlan nasal drops /
- baicalin /
- content
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