Study on quality control of Minuo Hair Solution
-
摘要: 目的 完善米诺生发搽剂的质量标准，以保证产品质量。 方法 采用薄层色谱法、紫外-可见光谱法分别对制剂中的当归、赤芍、白鲜皮及米诺地尔进行定性鉴别；采用一阶导数光谱法对制剂中的米诺地尔进行含量测定。 结果 薄层色谱斑点清晰，在与对照品或对照药材相应的位置上显相同颜色的斑点，阴性对照无干扰；紫外-可见光谱鉴别中，米诺地尔在（230±3）、（262±3）、（286±3）nm波长处有最大吸收峰；一阶导数光谱法含量测定中，米诺地尔在6.2～18.6μg/ml（r=0.9997）范围内线性关系良好；平均回收率为99.4%，RSD为0.22%。 结论 本方法操作简便，准确度高，重复性好，能全面、有效地控制该制剂的质量。Abstract: Objective To improve the quality standard of Minuo Hair Solution and to ensure product quality. Methods Thin layer chromatography (TLC) and UV-visible spectroscopy (UV-Vis) method was used for the qualitative identification of Angelica sinensis, Paeoniae Radix Rubra, Dictamni Cortex and minoxidil respectively. The Quantitive determination of minoxidil was completed by first order derivative spectroscopy. Results The spots in TLC were clear without inference. And the same color spots appeared in the corresponding position of references and reference drugs. The UV-Vis spectral absorption of minoxidil were in (230±3), (262±3),(286±3) nm. The linear range of minoxidil was obtained between 6.2 and 18.6 μg/ml (r=0.9997). The average recovery was 99.4% (RSD=0.22%). Conclusion The method is simple,accurate and reproducible,and can be effectively used to control the quality of Minuo Hair Solution.
-
Key words:
- Minuo Hair Solution /
- minoxidil /
- Angelica sinensis /
- Paeoniae Radix Rubra /
- Dictamni Cortex /
- quality standard
-
[1] 曹蕾,王磊,杜旭峰,等. 雄激素性秃发治疗研究进展[J]. 临床皮肤科杂志,2015,44(2):129-132. [2] 叶艳婷,曹慧,李水凤,等. 5%米诺地尔治疗33例女性型脱发治疗前后的临床和皮肤镜观察[J]. 临床皮肤科杂志,2014,43(12):739-741. [3] 邓伟. 外用米诺地尔治疗女性雄激素性脱发的系统评价[J]. 四川医学,2012,33(2):261-264. [4] 国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社,2010:附录Ⅵ B, 附录ⅤA. [5] 刘宁.消栓口服液的薄层色谱法鉴别研究[J]. 药物分析杂志,2008,28(11):1937-1939. [6] 张晓明,何晓英. 冠心康颗粒质量标准研究[J]. 中国药事,2010,24(4):380-382. [7] 孟德胜,傅若秋,吴畏. 5%米诺地尔搽剂的制备及稳定性[J]. 药学服务与研究,2010,10(3):189-190. [8] 朱利霞,张汉扬,杨春云,等. 白鲜皮药材的质量标准研究[J]. 中华中医药学刊,2015,33(1):155-159. [9] 谢苗,蓝海. 天然药物分析方法的研究进展[J]. 安徽农业科学,2014,42(29):10059-10062,10065. -
点击查看大图
计量
- 文章访问数: 3403
- HTML全文浏览量: 330
- PDF下载量: 621
- 被引次数: 0
下载:
