High purity preparation and identification of three phthalide compounds from Apium graveolens
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摘要: 目的 分离和鉴定芹菜籽中苯酞类化合物。 方法 采用硅胶柱色谱、制备薄层色谱方法分离纯化苯酞类化学成分，用波谱分析技术鉴定化合物结构。 结果 从芹菜籽中分离制备得到3种主要的苯酞类化合物，分别鉴定为芹菜甲素、芹菜乙素和新蛇床内酯。 结论 采用该法制备的样品纯度大于98%，可作为对照品使用。Abstract: Objective Isolation, purification and structure identification of phthalides from Apium graveolens L. Methods Silica gel column and preparative TLC chromatography were used to isolate and purify the phthalide compounds. The structures of those compounds were determined through spectral analysis. Results Three pure compounds were identified as 3-n-butylphthalide, sedanenolide and sedanolide. Conclusion Three pure phthalide compounds can be used as analytical reference standard for quantitative analysis of A. graveolens.
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Key words:
- Apium graveolens /
- 3-n-butylphthalide /
- sedanenolide /
- sedanolide /
- structure identification
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