吲哒帕胺、维拉帕米对兔心肌再灌注损伤保护机制的探讨
The protective effect of indapndde and verapamil in myocardial ischemia and reperfusion injury in rabbit Langendorff heart
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摘要: 应用离体兔心Langendorff灌注模型,探讨吲哒帕胺、维拉帕米对心肌缺血/再灌注损伤的保护机制。离体免心肌缺血40min后,分别取再灌注15、30、60、360min洗脱液,测定超氧化物歧化酶(SOD)、脂质过氧化物丙二醛(MDA);测定冠脉流量和冠脉阻力及静脉血血小板聚集率。结果表明,吲哒帕胺、维拉帕米均具有清除氧自由基、扩张冠状动脉作用;吲哒帕胺还具有抗血小板聚集的作用。Abstract: The animal medels of isolated myochoial pehschemic reperfusion injury in rabbits using Langendorff apparatus were used to inquire into the protective effect of indapamide and verapamil. Methods: Twenty - one male rabbits were randomly divided into three groups (n=7 in each): control group, verapamil group and indapamide group. Isolated heaxts were reperfused after 40 min of global no - flow ischemia, Reperfusion oulflow fluid were collected at 15 min,30 min 60 min and 300 min after postischemic reperfusion in order to measure SOD and MDA. Coronary flow, coronary resistance and platelet aggregation rate were tested. Results and Conclusion: The protective effect of indapamide and addition,apamil amy include: (1 )antioxide and free - radical scavenging; (2) dilating coroner artery. In addition, indapamide can decrease platelet aggregation rate.
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Key words:
- reperfusion injury /
- cardioprotection /
- free radical /
- platelet aggregatom rate
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