高效液相色谱法测定复方丹参片丹参酮ⅡA含量
Determination of tanshinone ⅡA in compound tanshin tablet by HPLC
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摘要: 目的 建立测定复方丹参片中丹参酮ⅡA含量的高效液相法。方法 色谱条件:色谱柱Spherisorb C18(5μ,200mm×4.6mm)色谱柱;流动相:甲醇-水(85:15);检测波长:270nm;流速:1ml/min.结果 丹参酮ⅡA在8～180ng范围内线性关系良好(r=0.9999),最低检出限1.5ng,阴性样品对测定无干扰,溶液在24h内保持稳定,加样回收率95%以上。结论 该方法快速、准确,可用于复方丹参片中丹参酮ⅡA的鉴别和含量测定。Abstract: OBJECTIVE To develop a method of HPLC to determine the content of tanshinone ⅡA in compound tanshin tablet,METHODS Spherisorb C18 (5μ,200mm×4.6mm)was used,mobile phase wasmethanol-waver(85:15).The detection wanelength was 270nm.RESULTS The linear ranges of determination of tanshinone ⅡA was 8～180ng(r=0.9999),the average recovery rate was 99.5%.CONCLUTION:This method may be used for determination tanshinone ⅡA in compound tanshin tablet.
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Key words:
- HPLC /
- tanshin /
- tanshinoneⅡA
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