反相高效液相色谱法测定消炎颗粒中黄芩苷的含量
Determination of baicalin in Xiaoyan granules by RP-HPLC
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摘要: 目的 测定消炎颗粒中黄芩苷的含量。方法 RP-HPLC法,采用C18分析柱,甲醇-0.2%磷酸(60:40)为流动相,检测波长为280nm。结果 该法平均回收率为100.64%,RSD=1.69%(n=5)。结论 本法快速、准确,样品处理简便易行,重现性好。Abstract: OBJECTIVE To determine the content of baicalin in Xiaoyan granules. METHODS Re verse-phase high performance liquid chromatography was established. A C18 column(250mmX 4. 6mm,5um)with mobile phase of MeOH:0. 2%phosphat(60:40)was used. The detection wavelength was 280nm. RESULTS:The mean recoveries were 100.64%(RSD-1.69%). CONCLUSION The method was rapid, sensitive and accurate.
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Key words:
- RP-HPLC /
- Xiaoyan granules /
- baicalin
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