柱前衍生化HPLC法测定人纤维蛋白原中精氨酸的含量
Determination of arginine in human fibrinogen by HPLC with precolumn derivatization
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摘要: 目的: 建立人纤维蛋白原中精氨酸的含量测定方法。 方法: 以丙氨酸为内标, 2, 4-二硝基氟苯(DN-FB)为柱前衍生剂, 50%乙腈-0.05mol/L醋酸盐缓冲液(35∶65)为流动相, 紫外362nm处检测。 结果: 精氨酸在0.16~0.80mg/mL浓度范围内线性关系良好(r=0.9998), 平均空白加样回收率为102.4%, RSD为1.4%。 结论: 方法准确、简便, 费用低, 可用于人纤维蛋白原中精氨酸含量的控制。Abstract: Objective: To develop a method for determination of arginine in human fibrinogen. Methods: The sample was mixed with alanine as the internal standard, derived by 2, 4-dinitrofluorobenzene.The mobil phase was composed of 50% acetonitrile-0.05 mol/L acetate buffer(35∶65).The UV detection wavelength was 362nm. Results: There was a good linear relationship with the range of 0.16~0.06mg/mL(r=0.9998), average recovery of arginine was 102.4%, RSD was 1.4%. Conclusion: This method is accu-rate, simple, cheap, and can be used for determination of arginine in human fibrinogen.
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Key words:
- arginine /
- alanine, HPLC /
- derivation /
- 2, 4-dinitrofluorobenzene /
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