摘要:
目的: 建立一种反相高效液相色谱分析方法,同时测定小柴胡汤中黄芩苷和甘草酸的含量。 方法: 采用Agilent Zorbax SB C18柱(4.6mm×250mm,5m),以乙腈-0.01%磷酸溶液为流动相,梯度洗脱,流速1.0mL/min.柱温25℃,检测波长为250nm。 结果: 黄芩苷和甘草酸单铵盐在50min内即实现良好分离,黄芩苷浓度在12.66~405.0g/mL线性范围内呈良好的线性关系,其回归方程为:Y=19.726X+13.972,r= 0.9998。甘草酸在1.988~63.60g/mL的线性范围内呈良好的线性关系,其回归方程为:Y=10.765X+ 6.6433,r=0.9997。两种成分精密度试验RSD<1%;48h内稳定性试验RSD<1%;加样回收率试验RSD<2%。 结论: 该方法简便快速、准确可靠,能同时测定小柴胡汤中黄芩苷及甘草酸两种有效成分的含量,为有效控制小柴胡汤的质量提供参考。
Abstract:
Objective: To establish a method for simultaneous determination of baicalin,glycyrrbizic acid in Xiaochaihu decoction by RP-HPLC. Methods: A Agilent Zorbax SB C18(4.6mm×250mm,5m) was used with a mobile phase of acetonitrile-0.01% H3 PO4 in gradient elution,The flow rate was 1.0mL/min and the detection wavelength was set at 250nm. Results: The two constituents were separated within 50 min.The linearity was obtained over 12.66~405.0g/mL(r=0.9998)for baicalin,1.988~63.60 g/mL(r =0.9997) for glycyrrhizic acid respectively.The RSD of precision and stability of the sample was less than 1% in 48 hours.The RSD of the average recovery was less than 2%. Conclusion: The method is rapid,simple and accurate,and it can be suitable for the simulta- neous determination of baicalin,glycyrrhizic acid in Xiaochaihu decoction and the quality control of Xiaochaihu decoction.