摘要:
目的: 用高效液相色谱法测定石韦中木犀草素的含量。 方法: 采用Shimadzu C18柱(150mm×4.6mm,5μm),流动相为甲醇-0.2%H3PO4溶液(50:50),流速1.0mL/min,检测波长254nm,柱温室温,用外标法定量,测定石韦中木犀草素的含量。 结果: 木犀草素的线性范围0.0325~0.391μg,r=0.9999,回收率95.85%,RSD为0.27%。 结论: 该方法简便,快速,线性关系良好,适合作为石韦中木犀草素含量测定的方法。
Abstract:
Objective: To determine the content of luteolin in Pyrrosia lingua by HPLC. Methods: The HPLC method was used to deter- mine the content of luteolin from Pyrrosia lingua.The analysis was carried out on Shimadzu C18 colunm(150mm×4.6mm,5μm).The mobile phase was MeOH-0.2% H3 PO4(50:50).Flow-rate was 1.0mL/min.Wave-length was 254nm.Temperature was room temperature. Results: The method was simple and had a good linear relationship.The liner range of luteolin is 0.40~2.00μg and gave a correlation (r)of 0.999 6.The recovery,was 95.85%,RSD 0.27%. Conclusion: The method was easy to determine the content of luteolin from Pyrrosia lingua.