麝香保心丸对裸小鼠人结肠癌肝转移模型促血管新生因子及肿瘤生长的影响
Effects of Shexiang Baoxin pill on Preangiogenesis factors and tumor growth of human colon cancer liver metastasis in nude mice
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摘要: 目的:探讨麝香保心丸对肿瘤组织血管新生的影响。方法:BALB/C裸小鼠40只,建立人结肠癌LoVo癌肝转移模型。随机分为4组:麝香保心丸大剂量组,中剂量组,小剂量组和模型组。分别进行相应干预,用免疫组化检测VEGF、bFGF、Ⅷ因子相关抗原。结果:麝香保心丸各剂量组肿瘤组织血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)、Ⅷ因子相关抗原(Ⅷ-R Ag)等均明显低于模型组(P<0.05)。结论:麝香保心丸可明显抑制人结肠癌LoVo裸小鼠癌肝转移肿瘤组织的血管生成。Abstract: Objective :To study the angiogenesis effects of Shexiang Baoxin pill on cancer. Methods :The model of liver metastasis of colorectal cancer was established on 40 BALB/C nude mice which were xenotransplanted through intraspleen injection of LoVo cells,a human colon cancer cell line.These mice were divided into four groups randomly as following: the high-dose-group with Shexiang Baoxin Pill,the medium-dose-group with Shexiang Baoxin Pill,the low-dose-group with Shexiang Baoxin Pill and the model group.The rats of each group were given intragastric administration every day.After 35 days of treatment,vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF) and Ⅷ-R Ag were messured by immunohistochemical staining. Results :The contents of VEGF,bFGF and Ⅷ-R Ag in herb groups were significantly lower than that of the model group(P<0.05). Conclusion : Shexiang Baoxin Pill can obviously inhibit the angiogeneses in cancer tissues.
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Key words:
- Shexiang Baoxin Pill /
- tumor /
- angiogenesis
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