高效液相色谱法测定银仙通肺宝颗粒中岩白菜素的含量
Determination of bergeninum in Yinxian Tongfeibao granules by HPLC
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摘要: 目的: 应用高效液相色谱法测定银仙通肺宝中岩白菜素的含量。 方法: 色谱柱为Zorbax Eclipse XDB-C18柱(4.6mm×250mm,5μm),流动相为甲醇-水-冰醋酸(18:81:1),检测波长275nm,流速0.8mL/min,柱温30℃。 结果: 岩白菜素在0.432.15μg范围内呈良好的线性关系;平均加样回收率为98.33%;RSD为2.68%。 结论: 本方法检测快速,定量准确,重现性好,可用于岩白菜素的含量测定。Abstract: Objective: To develop a HPLC method for determining bergeninum in Yinxian Tongfeibao granules. Methods: Zorbax Eclipse XDB-C18 column was used and methanol-water-acetic acid glacial(18-81-1) was used as mobil phase,detection wavelegth was 275 nm,the flow rate was 0.8 mL/min,column temperature was 30 ℃. Results: There was a good linear relationship in the range of 0.43-2.15 μg, r=0.999 88.The average recovery was 98.33% with RSD=2.68%. Conclusion: The method is rapid ,accurate and repeatable.It can be applied to an effective control of the quantity of Yinxian Tongfeibao granules.
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Key words:
- HPLC /
- Yinxian Tongfeibao /
- granules /
- bergeninum
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