摘要:
目的: 建立HPLC同时测定丹参酮含片中隐丹参酮和丹参酮ⅡA含量的方法。 方法: 采用分析色谱柱,用十八烷基硅烷键合硅胶为填充剂（Agilent ZORBAX Eclipse XDB-C18,4.6×150 mm,5μm）。流动相:乙腈-水（55:45）,流速:1.0 mL/min,检测波长254 nm。 结果: 隐丹参酮和丹参酮ⅡA的回归方程分别为:Y=0.0002709X+0.001090和Y=0.0002556X+0.001055,r均为0.9999。线性范围分别是0.036001.440μg和0.030001.200μg。平均回收率和RSD分别为101.7%、0.28%和100.8%、0.5%。 结论: 本方法操作简便,测定结果准确。
Abstract:
Objective: To develop a HPLC quantitative method for the determination of cryptotanshinone and tanshinone ⅡA in Danshentong buccal tablets. Methods: The chromatographic conditions include column C18(Agilent ZORBAX Eclipse XDB-C18,4.6×150 mm,5 μm),mobile phase:acetonitrile-water(55-45),flow rate 1.0 mL/min and detection wavelength 327 nm. Results: The regresstion equation of cryptotanshinone and tanshinone ⅡA was Y=0.000 270 9X+0.001 090,r=0.999 9,Y=0.000 255 6X+0.001 055,r=0.999 9.The linear range was 0.036 00～1.440 μg and 0.030 00～1.200 μg.The average recovery of cryptotanshinone and tanshinone ⅡA were 101.7%(RSD 0.28%),100.8%(RSD 0.50%)respectively. Conclusion: The method was sensitive,rapid and accurate.
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