高效液相色谱法测定氯霉素醇溶液中氯霉素的含量
Determination of chloramphenicol alcoholic solution by HPLC
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摘要: 目的: 建立高效液相色谱法测定氯霉素醇溶液中氯霉素的含量。 方法: 采用高效液相色谱法,色谱柱为ZORBAx 80A Extend-C18(4.6 mm×150 mm,5μm);以乙腈-0.1%庚烷磺酸钠溶液(25:75)为流动相;检测波长:278 nm;流速:1mL/min;柱温:35℃;进样量:10μL。 结果: 浓度在24.74~247.4μg/mL(r=0.9999)范围内具有良好线性关系,平均回收率为99.28%,RSD为0.27%(n=9)。 结论: 方法可靠、简单可行,可用于测定氯霉素醇溶液中氯霉素的含量。Abstract: Objective: To developed a method for determination of chloramphenicol alcoholic solution. Methods: HPLC method was carried out on a ZORBAX 80A Extend-C18 column(4.6 mm×150 mm,5μm) with UV detection at 278 nm.The mobile phase was acetonitrile-0.1% sodium heptanesulfonate(25:75) with a flow rate of 1.0 mL/min.The column temperture was 35℃and the inject volumes was 10μL.The content of chloramphenicol was calculated by external standardization method. Results: There was a good lin- ear relationship for chloramphenicol within the range of 24.74~247.4μg/mL(r=0.999 9).The average recovery was 99.28% and RSD was 0.27% (n=9). Conclusion:: TThe method is simple,specific and not interfered by excipients.It is accurate for quality control of chloramphenicol alcoholic solution.
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Key words:
- chloramphenicol /
- HPLC /
- alcoholic solution
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