摘要:
目的: 建立HPLC测定小儿润肺口服液中黄芩苷含量的方法。 方法: 色谱柱用十八烷基硅烷键合硅胶为固定相(Agilent ZORBAX Eclipse XDB-C18,4.6 mm×150 mm,5μm)。流动相为甲醇-水-磷酸(38:62 :0.5),流速:1 mL/min,检测波长278 mm。 结果: 黄芩苷的保留时间约为20.6 min,与相邻峰的分离度大于1.5。以峰面积(X)对进样浓度(Y,μg/mL)线性回归,回归方程:Y=0.060 47X+1.131,r=0.999 9,线性范围:62.84～314.2μg/mL,加样回收率为103.3%,RSD为0.43%。 结论: 本法测定结果准确,且操作简便,精密度好,可以用于小儿润肺口服液中黄芩苷的含量测定。
Abstract:
Objective: To develop an HPLC quantitative method for the determination of baicalin in Xiaoer Runfei Oral liquid. Methods: HPLC system consisted an Agilent C18column（ZORBAX Eclipse XDB-C18,4.6 mm×150 mm,5μm）and a mixture of methanol-water-phosphoric acid（38:62:0.5）as mobile phase.The flow rate was 1.0 mL/min and was monitored at 278 nm. Results: The retention time of baicalin was 20.6 min.The regress equation for baicalin was Y=0.060 47X+1.131,r=0.999 9,and the linear range was 62.84～314.2μg/mL.The average recovery of baicalin was（n=6）103.3%,RSD 0.43%. Conclusion: The method was sensitive,time-saving and accurate.
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