摘要:
目的:探讨锗(Germanium,Ge)对关节炎大鼠前列腺素E2(Prostaglandin E2,PGE2)的调节作用。方法:利用弗氏完全佐剂(Freund's complete adjuvant,FCA)诱导制造关节炎大鼠模型,用不同剂量的β-羧乙基锗倍半氧化物(carboxyethylgermanium sesquioxide,Ge-132)进行治疗。于造模前、造模后第3、6、9、12、15、18、21d观测每只大鼠的一般情况,计算体重增长率和足跖肿胀率。造模后21 d断头处死大鼠,测量组织内炎症介质PGE2的含量。结果:经过干预后,锗中、高剂量组及阳性药物组大鼠的食欲、活力、体重和足跖肿胀等状况优于模型组。组织内炎症介质PGE2的含量,锗中、高剂量组及阳性药物组均显著低于模型组(P<0.01),干预组中锗高剂量组最低,其次为锗中剂量和阳性药物组,锗低剂量组为最高。结论:各剂量的锗均可显著下调关节炎大鼠炎症介质PGE2,高剂量的锗效果优于吲哚美辛(indomethacin,IDMT),锗对关节炎大鼠的PGE2调节作用有剂量效应关系。
Abstract:
Objective :To investigate the regulatory effect of germanium(Ge) on PGE2 of the rheumatoid arthritic model. Methods :The rat model of rheumatoid arthritis(RA) induced by Freund's complete adjuvant(FCA) was interfered with different dosages of β-carboxyethylgermanium sesquioxide(Ge-132).The body weight and the paw volume of each rat model were measured at the 3rd,6th,12th,15th,18th and 21st days,and the weight growth rate and paw swelling rate were caculated.After rats was quickly beheaded at the 21st day,the contents of Prostaglandin E2(PGE2) in the tissue were measured. Results :Appetite,energy,weight gains and the paw volume of germanium with middle and high dosages and Indomethacin(IDMT) treated groups were better then those of the model group(P<0.01).Compared with the IDMT treated group,the level of tissue inflammatory mediator PGE2 in germanuium with middle and high dosages and the normal saline treated groups were significantly lower than that of the model group(P<0.01),and the germanuium with high dosage was the lowest one,while the germanuium with middle dosage and the IDMT treated groups were in the second place,and the germanuium with low dosage was the highest one among all the groups. Conclusion :All the dosages of germanuium were able to decrease PGE2 significantly by measuring the content of the inflammatory mediator;the high dosed germanuium gave better anti-inflammatory effects on the experimental RA rat model than IDMT.There was a relationship between the dose and enhancement effect of germanuium.
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