留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
x 关闭 永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用

陈迁,  陈孟莉,  叶兆波

downloadPDF
文章导航 > 药学实践与服务  > 2012 >  30(1): 25-28,31
陈迁, 陈孟莉, 叶兆波. 五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用[J]. 药学实践与服务, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
引用本文: 陈迁, 陈孟莉, 叶兆波. 五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用[J]. 药学实践与服务, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
shu
CHEN Qian, CHEN Meng-li, YE Zhao-bo. Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
Citation: CHEN Qian, CHEN Meng-li, YE Zhao-bo. Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
shu

五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用

doi: 10.3969/j.issn.1006-0111.2012.01.006
  • 1.

    中国人民解放军总医院临床药理研究室,北京 100853

  • 2.

    香港中文大学中医学院,香港

基金项目: 国家自然科学基金资助项目(81041030).

Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat

  • 1.

    Department of clinical pharmacology research, General Hospital of PLA, Beijing 100853 China

  • 2.

    College of traditional Chinese medicine , The Chinese University of Hong Kong , Hong Kong,China

  • 摘要: 目的 在前期研究工作的基础上,对具有肝脏保护作用的中药五子衍宗复方(WZ)进行进一步的分离,考察WZ复方提取物中总多糖(TPC)对慢性酒精中毒大鼠肝脏的保护作用并探讨其可能的作用机制。 方法 本实验采用改良的Lieber-DeCarli液体酒精饲料喂养致慢性酒精性肝损伤大鼠模型,应用TPC治疗,观察血清生化指标和病理学改变,考察对肝脏氧化应激、脂质过氧化指标和细胞因子TNF-α水平及TNF-α mRNA 表达影响。 结果 TPC能够显著降低升高的肝脏指数和血清ALT、AST水平,减轻肝脏组织病理学改变;减
    Abstract: Objective To investigate the protect effect and mechanism of total polysaccharides (TPC) isolated from 50% ethanol extract of Wuzi-Yanzong pill (WZ) against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic ethanol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator (Serum ALT, AST) and histopathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent hepatoprotective activities in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attributed to the antioxidant action and suppress TNF-α expression at protein and gene level.
  • [1] 庄 辉. ALD 的流行病学[J]. 中华肝脏病杂志, 2003, 11: 698.
    [2] 全国酒精性肝病调查协作组. 全国酒精性肝病的多中心调查分析[J]. 中华消化杂志 2007; 27: 231.
    [3] 中国药典[M]. 2005版1部. 2005: 364.
    [4] Chen ML, Tsai SH, Ip SP, et al. Long-term treatment with a "Yang-Invigorating" Chinese herbal formula, WuZi-YanZongWan, reduces mortality and liver oxidative damage in chronic alcohol-Intoxicated rats[J]. Rejuv Res, 2010, 13: 459.
    [5] Chen ML, Ip SP, Tsai SH, et al. Biochemical mechanism of Wu-Zi-Yan-Zong-Wan, a traditional Chinese herbal formula, against alcohol-induced oxidative damage in CYP2E1 cDNA-transfected HepG2 (E47) cells[J]. J Ethnopharmacol, 2010, 128: 116.
    [6] Ronis MJ, Hakkak R, Korourian S, et al. Alcoholic liver disease in rats fed ethanol as part of oral or intragastric low-carbohydrate liquid diets[J]. Exp Biol Med 2004; 229: 351.
    [7] Albano E. New concepts in the pathogenesis of alcoholic liver disease[J]. Expert Rev Gastroenterol Hepatol 2008; 2:749.
    [8] Arteel GE. Oxidants and antioxidants in chronic alcoholic liver disease[J]. Gastroenterology, 2003, 124: 778.
    [9] Hanck C, Rossol S, Bocker U, et al. Presence of plasma endotoxin is correlated with tumour necrosis factor receptor levels and disease activity in alcoholic cirrhosis[J]. Alcohol Alcoholism, 1998, 33:606.
    [10] Tilg H, Wilmer A, Vogel W, et al. Serum levels of cytokines in chronic liver diseases[J]. Gastroenterology, 1992, 103: 264.
    [11] Tilg H, Jalan R, Kaser A, et al. Anti-tumor necrosis factor-α monoclonal antibody therapy in severe alcoholic hepatitis[J]. J Hepatol, 2003, 38: 419.
    [12] Menon K, Stadheim L, Kamath P, et al. Safety and tolerability of etanercept in patients with alcoholic hepatitis[J]. Am J Gastroenterol, 2004, 99: 255.
    [13] Spahr L, Rubbia-Brandt L, Frossard J, et al. Combination of steroids with infliximab or placebo in severe alcoholic hepatitis: a randomized controlled pilot study[J]. J Hepatol, 2002, 37: 448.
  • [1] 毛智毅, 王筱燕, 陈晓颖, 汤逸斐.  度拉糖肽联合二甲双胍对肥胖型2型糖尿病患者机体代谢、体脂成分及血清脂肪因子的影响 . 药学实践与服务, 2024, 42(7): 305-309. doi: 10.12206/j.issn.2097-2024.202305032
    [2] 姜涛, 徐卫凡, 蒋益萍, 夏天爽, 辛海量.  巴戟天丸组方对Aβ损伤成骨细胞的作用及基于网络药理学的机制研究 . 药学实践与服务, 2024, 42(7): 285-290, 296. doi: 10.12206/j.issn.2097-2024.202305011
    [3] 赖立勇, 夏天爽, 徐圣焱, 蒋益萍, 岳小强, 辛海量.  中药青蒿抗氧化活性的谱效关系研究 . 药学实践与服务, 2024, 42(5): 203-210, 216. doi: 10.12206/j.issn.2097-2024.202211012
    [4] 邹思, 吴岩斌, 吴锦忠, 吴建国, 黄家兴.  虎奶菇菌核多糖功能化纳米硒抗疲劳功效研究 . 药学实践与服务, 2024, 42(10): 426-432. doi: 10.12206/j.issn.2097-2024.202206072
    [5] 张广雨, 杜晶, 刘梦珍, 朱丹妮, 闫慧, 刘冲.  新斯的明与山莨菪碱联合应用对肺型氧中毒的保护作用及其机制的研究 . 药学实践与服务, 2024, 42(10): 433-438, 444. doi: 10.12206/j.issn.2097-2024.202310049
    [6] 修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星.  全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 291-296. doi: 10.12206/j.issn.2097-2024.202312054
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  4372
  • HTML全文浏览量:  235
  • PDF下载量:  440
  • 被引次数: 0
出版历程
  • 收稿日期:  2011-08-11
  • 修回日期:  2011-10-14

五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用

doi: 10.3969/j.issn.1006-0111.2012.01.006
  • 1. 中国人民解放军总医院临床药理研究室,北京 100853
  • 2. 香港中文大学中医学院,香港
    • 基金项目:  国家自然科学基金资助项目(81041030).
  • 收稿日期: 2011-08-11
  • 修回日期: 2011-10-14

摘要: 目的 在前期研究工作的基础上,对具有肝脏保护作用的中药五子衍宗复方(WZ)进行进一步的分离,考察WZ复方提取物中总多糖(TPC)对慢性酒精中毒大鼠肝脏的保护作用并探讨其可能的作用机制。 方法 本实验采用改良的Lieber-DeCarli液体酒精饲料喂养致慢性酒精性肝损伤大鼠模型,应用TPC治疗,观察血清生化指标和病理学改变,考察对肝脏氧化应激、脂质过氧化指标和细胞因子TNF-α水平及TNF-α mRNA 表达影响。 结果 TPC能够显著降低升高的肝脏指数和血清ALT、AST水平,减轻肝脏组织病理学改变;减

English Abstract

陈迁, 陈孟莉, 叶兆波. 五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用[J]. 药学实践与服务, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
引用本文: 陈迁, 陈孟莉, 叶兆波. 五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用[J]. 药学实践与服务, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
shu
CHEN Qian, CHEN Meng-li, YE Zhao-bo. Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
Citation: CHEN Qian, CHEN Meng-li, YE Zhao-bo. Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 25-28,31. doi: 10.3969/j.issn.1006-0111.2012.01.006
shu

    全文HTML

参考文献 (13)

目录

    /

    返回文章
    返回

    版权所有：《药学实践与服务》编辑委员会

    主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

    电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    本系统由 北京仁和汇智信息技术有限公司 开发    百度统计