α-Glucosidase inhibitory activity of extracts from roots of Salacia amplifolia Merr.ex ChunF.C.
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摘要: 目的 研究阔叶五层龙根各提取物体外α-糖苷酶抑制活性。 方法 将阔叶五层龙根80%乙醇提取物依次用石油醚、乙酸乙酯、正丁醇萃取,得到4个不同极性部位,并对80%乙醇总提取物与4个不同极性部位进行α-葡萄糖苷酶和α-淀粉酶体外抑制活性筛选。 结果 阔叶五层龙根各提取物均具有一定的α-葡萄糖苷酶和α-淀粉酶体外抑制活性,其中对α-葡萄糖苷酶的抑制活性大小:正丁醇部位 > 石油醚部位 > 乙酸乙酯 > 总提取物;对α-淀粉酶的抑制活性大小:石油醚部位 > 正丁醇部位 > 总提取物 > 乙酸乙酯部位。 结论 阔叶五层龙根各提取物均具有较好的α-糖苷酶抑制活性和很好的开发价值。Abstract: Objective To investigate different extracts of Salacia amplifolia Merr.ex ChunF.C on inhibitory activity to α-glucosidase. Methods The 80% aqueous ethanol extracts from roots of S.amplifolia, which were partitioned sequentially with petroleum ether, EtOAc and n-BuOH respectively to yield four different fractions. And the inhibitory activity of these five extracts for the α-glucosidase and α-amylase were determined by in vitro experiments. Results Five kinds of extracts showed moderate inhibitory activity at the same time and close to acarbose to a certain degree.Inhibitory activity of α-glucosidase were, n-BuOH fraction > petroleum ether fraction > EtOAc fraction > total fraction.The inhibitory of α-amylase activity followed by petroleum ether fraction > n-BuOH fraction > total fraction > EtOAc fraction, respectively. Conclusion The extracts from S.amplifolia had good inhibitory activity on α-glucosidase, which could be exploited the diabetes drugs as α-glucosidases inhibitors in future.
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Key words:
- Salacia amplifolia /
- α-glucosidase /
- inhibitory activity
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