Effect of the curcumin on expression of IL-2 and IL-6 of hippocampus in pentylenetetrazol-induced epilepsy in rats
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摘要: 目的 探讨姜黄素抗癫GFDA3的作用机制。 方法 取健康成年雄性SD大鼠，连续腹腔注射戊四氮，诱发大鼠点燃致癫模型。空白组和模型组灌予生理盐水5 ml，1次/d，连续28 d。低剂量和高剂量姜黄素组分别灌予姜黄素100 mg/kg和200 mg/kg，1次/d，连续28 d；丙戊酸钠组灌予丙戊酸钠400 mg/kg，1次/d，连续28 d。治疗结束后，按照Racine的6级评分标准，观察癫GFDA3大鼠发作等级变化，用酶联免疫法（ELISA）检测海马区IL-2和IL-6表达水平的变化。 结果 姜黄素组的癫GFDA3大鼠惊厥发作等级降低。模型组IL-2和IL-6高表达，比空白组呈显著升高（P<0.05）。与模型组比较，姜黄素组大鼠海马区IL-2和IL-6的表达明显降低（P<0.05）；与低剂量姜黄素组相比，高剂量姜黄素组IL-2和IL-6水平降低更为明显（P<0.05）。高剂量姜黄素组与丙戊酸钠组比较，无显著差异（P>0.05）。 结论 姜黄素能降低癫GFDA3大鼠惊厥发作级别，具有一定的抗癫GFDA3作用，并且呈剂量依赖性，其可能机制是通过降低海马区IL-2和IL-6的表达发挥作用。Abstract: Objective To investigate the mechanism of anti-epileptic effect of the curcumin. Methods The SD rats were injected intraperitoneally with pentylenetetrazol kindling 25.0 mg/kg to induce a rat epilepsy model. All of the treatments were performed once a day continuously for 28 days. The rats in blank group and model group received 5 ml of normal saline. The rats in the high and low curcumin group were given 200 mg/kg and 100 mg/kg of curcumin once a day, respectively. The rats in the sodium valproate (VPA) group were given 400 mg/kg of VPA once a day by gavage. After treatment, the seizures level was recorded by using the Racine's six point grading scale, and the expression of IL-2 and IL-6 of hippocampus were detected by the enzyme linked immunoassay (ELISA). Results The seizures level was reduced by curcumin in epileptic rats. The expressions of IL-2 and IL-6 of the model group were significantly higher than those of the blank group (P<0.05), while those rats of the anti-epileptic groups, including high dose group and low dose group, were lower than those rats of the model group (P<0.05). When compared with the curcumin low dose group, the expression of IL-2 and IL-6 of curcumin high dose group is lower (P<0.05). There was no significant difference between the high dose curcumin group and VPA group (P>0.05). Conclusion The curcumin can reduce the seizure level in rats, it shows some anti-epileptic effets and dose-dependently, which may be through down-regulating the expression of IL-2 and IL-6 in hippocampus.
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[1] Iori V, Frigerio F, Vezzani A. Modulation of neuronal excitability by immune mediators in epilepsy[J]. Curr Opin Pharmacol,2015,26:118-123. [2] Liu ZS,Wang QW,Wang FL, et al. Serum cytokine levels are altered in patients with west syndrome[J]. Brain Dev,2001,23(7):548-551. [3] 向赟,董大翠,刘庆莹,等.马桑内酯致GFDA3大鼠海马IL-2免疫反应性的变化[J].江汉大学学报(自然科学版),2003,31(2):17-21. [4] RavizzaT, Rizzi M, Perego C, et a1. Inflammatory response and glia activation in developing rat hippoeampus after status epilepticus[J]. Epilepsia,2005,46(S5):113-117. [5] Lehtimki KA, Kernen T, Huhtala H, et al. Regulation of IL-6 system in cerebrospinal fluid and serum compartments by seizures:the effect of seizure type and duration[J]. J Neuroimmunol.2004,152(1-2):121-125. [6] Racine RJ. Modification of seizure activity by electrical stimulation Ⅱ. motor seizure[J]. Electroencephalogr Clin Neurophysiol,1972,32(3):281-294. [7] 文帅,梁日生,杨卫忠. 姜黄素神经保护作用与癫GFDA3[J]. 国际神经病学神经外科学杂志,2010,37(1):46-49. [8] Shin HJ, Lee JY, Son E, et al. Curcumin attenuates the kainic acid-induced hippocampal cell death in the mice[J]. Neurosci Lett,2007,416(1):49-54. [9] Agarwal NB, Jain S, Agarwal NK, et al.Modulation of pentylenetetrazole-induced kindling andoxidative stress by curcumin in mice[J]. Phytomedicine,2011, 18(8-9):756-759. [10] Mehla J, Reeta KH, Gupta P, et al. Protective effects of curcumin against seizares and congnitive impairment in a pentylenetetrazole-kindled epileptic rat model[J].Life Sci, 2010, 87(19-22):596-603. -
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